Question

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design an experiment to determine the concentration of HbA1c in the blood sample by following these six steps

Step 1, pick one differentiating characteristic

Step2 how will u probe that differentiating characteristic

Step3 tell the anticipated response to the probe

step4 measure the response to the probe

step 5 how will u interpret the response to the probe to obtain the desired information

step6 tell the likely interference with the method you have chosen.

you have to design experiment to tell the concentration of HbA1c

Answer 1

HbA1c is a glycated haemoglobin and it is used to check diabetes. When haemoglobin combines with glucose in the blood, becomes glycated haemoglobin. The  amount of sugar level is  directly proportional to amount  glycated haemoglobin.  Glycated haemoglobin (or HbA1c) can be measured before the renewation of RBC

The differentiating characteristic of HbA1c is to indicate prediabetes or diabetes so it is a indicator of diabetes.

The  reading of HbA1c can be taken from finger or arm blood , and it can be expressed as a percentage (DCCT unit) or mmol/mol (IFCC unit). The reading in the table given below gives us information about how  average blood sugar levels in mmol/L is translated into HbA1c readings, and vice versa.

		HbA1c (%) HbA1c (mmol/mol) Ave. Blood Glucose (mmol/L) 13 119 18 mmol/L 12 108 17 mmol/L 11 97 15 mmol/L 10 86 13 mmol/L 9 75 12 mmol/L 8 64 10 mmol/L 7 53 8 mmol/L 6 42 7 mmol/L 5 31 5 mmol/L
		HbA1c and blood sugar levels monitering can help to  improve blood sugar control. It can lower the risk of developing diabetes and its long-term complications. An UV-Vis spectroscopy method is  used to measure  Hb concentration before the hemolysate sample is applied to biosensors to take HbA1c readings. FcBA-based biosensors used for the detection of HbA1c.  HbA1c antibody binds wijh the HbA1c through the  micro immunosensor, sensor can detect the detect the concentration of HbA1c and Hb to obtain HbA1c level.