Question

Your qPCR quantification results show that you have recovered a total of 30ng in 200µl of AE buffer. First, calculate the concentration of your DNA solution. Second, calculate the volume of DNA to add to the PCR reaction if the total amount required is 2ng. please show all the answer steps.

Answer 1

1. Concentration of Dna solution = 30 X 10^-9 g / 200 X 10^-6 l

                                                    = 0.15 X 10^-9 X 10⁶ g/l

                                                    = 0.15 X 10^-3 g/l

                                                    = 150 g/l or 150 µg / µl

2. 30 X 10^-9 g contains ------------ 200 X 10^-6 l

     2 X 10^-9 g contains --------------     X liter

X = (2 X 10^-9 g X 200 X 10^-6 l ) / 30 X 10^-9 g

X= 400 X 10^-6 / 30l

X= 400 X 10^-6 / 30l

X = 13.33 µl required for PCR reaction