Question

In: Biology

compare and contrast detecting GMOs by PCR and ELISA

compare and contrast detecting GMOs by PCR and ELISA

Solutions

Expert Solution

The genetically modified organisms can be detected based on two methods:-

  • DNA based method
  • Protein based method

PCR is used for DNA based detection.

ELISA is used for protein based detection.

Comparison is shown in the following table:-

PCR ELISA
1. Detect the genome of the GMO. 1) Detecte the proteins expressed by the GMO.
2. Qualitatively measure whether GMO is present in the sample by targetting multiple genes. And also quantitatively measures the amount of transgene DNA present. 2. Antibodies specifically bind to genetically engineered protein and thus detect the expression of that protein by GMO.
3. Uses fluorescent dyes for detection. 3. Uses enzyme for detection.
4. It can amplify DNA so even a very small sample of DNA can be studied in GMO. 4. It is not used for amplification so small sample cannot be studied.
5. More sensitive. 5. Less sensitive.
6. Required Gel electrophoresis for visualisation. 7. The colour change to either activation or production of chromogen is detected by Spectrophotometry.

gladiator answered 3 years ago
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