Question

In: Biology

The ubiquitin protein from the malaria parasite was treated with chymotrypsin, and the resulting fragments were...

The ubiquitin protein from the malaria parasite was treated with chymotrypsin, and the resulting fragments were sequenced. A second sample of the polypeptide was treated with trypsin, and the fragments were sequenced. What is the sequence of the polypeptide?

Chymotrypsin fragments:

AGKQLEDGRTLSDY

IPPDQQRLIF

VKTLTGKTITLDVEPSDTEIN-VKAKIQDKEGI

NIQKESTLHLVLRLRGGMQIF

Trypsin Fragments:

LR

AK

EGI

IQDK

LIFAGK

QLEDGR

TLTGK

IPPDQQR

GGMQIFVK

TLSDYNIQK

ESTLHLVLR

Expert Solution

The sequence is

IPPDQQRLIF AGKQLEDGRTLSDY NIQKESTLHLVLRLRGGMQIF VKTLTGKTITLDVEPSDTEIN-VKAKIQDKEGI

Chymotrypsin cleavage site is
NH-R-R'-CO
R= phenylalanine (F), Tyrosine (Y), tryptophan (W)
R' is not proline (P)
The cleavage site of trypsin is
NH-R-CO
R= Lysine (K) or arginine (R)
In both the digestion we can see a fragment snding with Isoleucine (I). It is not a cleavage site of both the enzymes. Hence it is considered as the carboxy terminal of the polypeptide. Using this fragment (VKTLTGKTITLDVEPSDTEIN-VKAKIQDKEGI) we shall predict the sequence.

We shall consider the first 2 amino acid "VK" in VKTLTGKTITLDVEPSDTEIN-VKAKIQDKEGI for our analysis. Look for sequence ending with VK in trypsin.
We have GGMQIFVK. Now look for this sequence QIF in carboxy terminus of chymotrypsin fragments. We have this NIQKESTLHLVLRLRGGMQIF. Hence this fragment will come before the terminal sequence.

NIQKESTLHLVLRLRGGMQIF VKTLTGKTITLDVEPSDTEIN-VKAKIQDKEGI

Again look for NIQK in the trypsin fragment. We have TLSDYNIQK. Look for TLSDY in chymotrypsin fragments. We have AGKQLEDGRTLSDY.

The sequence becomes
AGKQLEDGRTLSDY NIQKESTLHLVLRLRGGMQIF VKTLTGKTITLDVEPSDTEIN-VKAKIQDKEGI

Finally look for AGK in trypsin fragments and it is LIFAGK. Look for LIF in chymotrypsin fragments and it is IPPDQQRLIF.

Hence the answer is IPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLDVEPSDTEIN-VKAKIQDKEGI

gladiator answered 1 year ago

. In a randomized controlled trial, insecticide-treated bednets were tested as a way to reduce malaria....

. In a randomized controlled trial, insecticide-treated bednets were tested as a way to reduce malaria. Among 332 infants using bednets, 15 developed malaria. Among 270 infants not using bednets, 22 developed malaria. Use a 0.05 significance level to test the claim that the incidence of malaria is lower for infants using bednets. Do the bednets appear to be effective? Conduct the hypothesis test by using the results from the given display. Difference= p(1)-p(2) Estimated for difference: -0.0363008 95% upper...

In a randomized controlled trial, insecticide-treated bednets were tested as a way to reduce malaria. Among...

In a randomized controlled trial, insecticide-treated bednets were tested as a way to reduce malaria. Among 322 infants using bednets, 13 developed malaria. Among 276 infants not using bednets, 29 developed malaria. Use a 0.05 significance level to test the claim that the incidence of malaria is lower for infants using bednets. Do the bednets appear to be effective? Conduct the hypothesis test by using the results from the given display. please show me how to do on ti-84 Difference=p(1)minus−p(2)...

what is the missing part of problems resulting from excessive consumption of protein

A hybrid protein resulting from the fusion of two chromosomes (chromosome 22 and chromosome 9) following...

A hybrid protein resulting from the fusion of two chromosomes (chromosome 22 and chromosome 9) following a translocation can result in this type of cancer Question 30 1) Chronic Myeloid Leukemia 2) Lung Cancer 3) Breast Cancer 4) Familial adenomatous polyposis (FAP) 5) Hereditary nonpolyposis colon cancer (HNPCC) The ability of cancer cells to move to different tissues is known as Question 33 1) Dysplasia 2) Angiogenesis 3) malignancy 4) Metaplasia 5) Metastasis

Mr. Johnson is a 52-year-old male being treated for hypovolemic shock resulting from bleeding injuries received...

Mr. Johnson is a 52-year-old male being treated for hypovolemic shock resulting from bleeding injuries received in a motor vehicle accident. Mr. Johnson has a decrease in his MAP of 12 mm Hg from his baseline reading, increased heart rate, decreased pulse pressure, decreased urine output, increased potassium level, and mild acidosis. He states, “I am so thirsty.” Describe the pathophysiology of sharp Mr. Johnson is presenting. Describe the priority treatments for Mr. Johnson providing explanation of how and why...

1. You were trying to produce a hydrophilic protein from E coli, but the protein you...

1. You were trying to produce a hydrophilic protein from E coli, but the protein you produced is inactive (misfolded) due to a disulfide bond formed between two cysteine residues that are not found in the native form. Your professor suggests you to co-express this protein with a folding assistant enzyme to improve its activity. (a) Which enzyme is it? (0.4 pt) (b) What are the functions of this enzyme? Please describe two aspects and indicate which sub-form execute which...

The following data are from 10 individuals that were treated with 2 different drugs that are...

The following data are from 10 individuals that were treated with 2 different drugs that are said to reduce acid in the stomach. Below are the pH’s of the stomach contents after giving the drugs to each patient. Tell me if one of the drugs are more effective than the other. Remember that a low pH is more acidic. Pepcid Prilosec 2.3 3.1 2.4 2.7 2.7 3.1 3.0 3.2 3.1 2.1

Think about the last time that you (1) were treated unfairly because you differed from a...

Think about the last time that you (1) were treated unfairly because you differed from a decision maker on a particular dimension of diversity or (2) observed someone else being treated unfairly because that person differed from a decision maker on a particular dimension of diversity. Then answer these questions: 1. Why do you think the decision maker acted unfairly in this situation? 2. In what ways, if any, were biases, stereotypes, or overt discrimination involved in this situation? 3....

6. The p53 tumor suppressor gene was isolated from a tumor where p53 protein levels were...

6. The p53 tumor suppressor gene was isolated from a tumor where p53 protein levels were very high. This was likely due to dominant-acting mutant that inactivated all p53 tetramers and blocked the tumor suppressing effects of wild type p53. A) True B) False 7. Increased gene expression from the Ink4A locus leads to inhibition of cyclin E-CDK2. A) True B) False 8. In colon cancer progression, the first driver mutation is commonly a mutation to the KRas gene. A)...