Question

Digestive tract and digestive enzyme mapping:

For both example's one and two and three:

Example 1: C-ILE-LEU-VAL-ASP-GLU-CYS-TRP-GLY-LYS-PHE-ARG-N

Example 2: C-CYS-TYR-ILE-GLN-ASN-CYS-PRO-LEU-GLY-N

Example 3: C-CYS-LYS-GLU-ASP-TRP-ASP-GLY-LYS-PRO-PHE-SER-ALA-N

1. take the amino acid chain in both examples and determine what digestive enzyme would be used as the protein becomes catabolized throughout the digestive tract

2. list the enzymes that will react with each amino in the protein chain

3. be sure to state where in the digestive system each phase of protein enzymatic gestation is happening.

4. putting the sequence of protein enzymatic digestion in order of movement of the protein through the digestive tract would be best for comprehension.

Answer 1

enzyme	action	location
aminopeptidase	on oligopeptides, they cleaves one aminoacid from the amine end	brush borders of small intestine
carboxypeptidase	on oligopeptides, they cleaves one aminoacid from the carboxyl end	lumen of small intestine
chymotrypsin	on polypeptide , cleaves carboxyl end of leucine, phenylalanine, tryptophan and tyrosin	lumen of small intestine
dipeptidase	cleaves dipeptide	brush borders of small intestine
pancreatic elastase	on polypeptide, cleaves carboxyl end of alanine, glycine, isoleucine and valine	lumen of small intestine
pepsin	protein cleaves amino end of tyrosin, phenylalanine and tryptophan	stomach
trypsin	on polypeptide, cleaves carboxyl end of arginine or lysine	lumen of small intestine