Roughly how many soluble proteins are there in E. coli? Refer back to your Isolation and...

Roughly how many soluble proteins are there in E. coli? Refer back to your Isolation and Purification of GFP lab. Describe what proteins should be present in each of your gel samples (crude, flow through, wash and elution samples). What is the known size of GFP from the literature? How will you determine the size of your GFP by SDS-PAGE?

Expert Solution

Several studies have investigated the proteome of E. coli. By 2006, 1,627 (38%) of the 4,237 open reading frames (ORFs) had been identified experimentally.^[54] The 4,639,221–base pair sequence of Escherichia coliK-12 is presented. Of 4288 protein-coding genes annotated, 38 percent have no attributed function. Comparison with five other sequenced microbes reveals ubiquitous as well as narrowly distributed gene families; many families of similar genes within E. coli are also evident. The largest family of paralogous proteins contains 80 ABC transporters. The genome as a whole is strikingly organized with respect to the local direction of replication; guanines, oligonucleotides possibly related to replication and recombination, and most genes are so oriented. The genome also contains insertion sequence (IS) elements, phage remnants, and many other patches of unusual composition indicating genome plasticity through horizontal transfer

gladiator answered 1 year ago

Explain how synthesis of ribosomal proteins in E. coli is regulated at the level of translation.

18. How can E. coli host cells be engineered so that complex proteins with a large...

18. How can E. coli host cells be engineered so that complex proteins with a large number of disulfide bonds are properly folded and therefore produced in an active form rather than as part of an inclusion body?

To study the molecular mechanism of DNA replication, you incubated soluble E. coli extracts with a...

To study the molecular mechanism of DNA replication, you incubated soluble E. coli extracts with a mixture of dATP, dTTP, dGTP, and dCTP, all of which are labeled with the α-phosphate group. After a while, the incubation mixture was treated with trichloroacetic acid, which precipitate the DNA but not the nucleotide or very short oligonucleotides. The precipitate was collected and the extent of the radioactively label nucleotide incorporation into the DNA was determined. a. Would this assay identify DNA? Explain....

If the concentration of ATP in an E. coli cell is 4.8 mM, how many ATP...

If the concentration of ATP in an E. coli cell is 4.8 mM, how many ATP molecules are in a rod-shaped E. coli cell that is 2.1 micrometers long and 0.6 micrometers in diameter? Enter your answer to the nearest ones.

If the concentration of glucose inside an E. coli cell is 5.1 mM, how many glucose...

If the concentration of glucose inside an E. coli cell is 5.1 mM, how many glucose molecules are in the cell? Assume an E. coli cell to be a cylinder 1.9 micrometers long and 0.8 micrometers in diameter. Enter your answer to the nearest ones. I thought it was 3.0*106 but it was wrong.

The E. coli trp operon encodes proteins involved in the biosynthesis of the amino acid tryptophan....

The E. coli trp operon encodes proteins involved in the biosynthesis of the amino acid tryptophan. Control of trp operon expression is mediated by two distinct regulatory mechanisms. Describe these two regulatory mechanisms in detail. Note Please give detail and comprehensive answer .

E. coli often comes to mind when reading about proteins and discussing them and it often...

E. coli often comes to mind when reading about proteins and discussing them and it often makes headlines in the news. My challenge for this chapter's discussion topic is this: find a recent (within the last month) news article that involves E. coli and share it with the class. Why does it seem that this bacteria is always in the news? Remember, this chapter is the protein chapter, so why is E. coli so important to proteins and vice versa?...

a) Describe how you could determine quantitate (determine how many) E. coli cells have been transformed?

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Using your data and the fact that plants are roughly 44.5% carbon, calculate how many days...

Using your data and the fact that plants are roughly 44.5% carbon, calculate how many days it would take for all of the carbon in your leaves to be respired if microbes continue to metabolize at the rate you measured. Now, think back to a just-fallen leaf outside. Considering how many days you estimate it would take your leaves to disappear, do you think there are enough days that are moist and warm enough to allow complete metabolism of leaves...

Your lab is studying gene expression of the tryptophan operon in the bacterium E. coli. In...

Your lab is studying gene expression of the tryptophan operon in the bacterium E. coli. In order to facilitate this study, you have cloned the Trp-operon, excised the five coding genes (TrpA, B, C, D, and E), and replaced them with a copy of the reporter gene GFP (green fluorescent protein). The resulting recombinant DNA was inserted into an F' plasmid, and used to transform a number of different strains of E. coli. For each of the scenarios listed below,...

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