Question

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To prepare DNA template with the concentration of 10 ng/μl:

Zach should add ____ μl of the original soil DNA (conc.: 250 ng/μl) and ______ μl of water into a tube to have final volume of 100 μl of the soil DNA that has the concentration of 10 ng/μl.

Answer 1

To prepare DNA template with the concentration of 10 ng/μl:

Zach should add 4 μl of the original soil DNA (conc.: 250 ng/μl= Stock) and 96 μl of water into a tube to have a final volume of 100 μl of the soil DNA that has the concentration of 10 ng/μl.

Concentration of DNA Stock =250 ng/μl

Required ethanol concentration (final concentration)            =10 ng/μl of 100 μl

M₁V₁    (Concentrated)                          =       M₂V₂ (Diluted)

250 ng/μl (Stock)        *          xul (?)     =   10 ng/μl (Final concentration we need) * 100 μl (volume we need)

xul =   10 ng/μl *100 μl / 250 ng/μl

=       4 μl

According to dilution principle to get 100 μl of 10 ng/ μl of DNA we should have to take 4 μl of 250 ng/μl DNA Stock and add 96 μl of water (i.e. 4 μl of 250 ng/μl Stock and makeup it into 100 μl with water).