Question

Q2. Below are the results of a manual reticulocyte count. Calculate the relative reticulocyte
count. Show calculation.
1
st slide = 15 reticulocytes counted in 1000 cells
2
nd slide = 17 reticulocytes counted in 1000 cells
Q3. The patient in Question 2 above is an adult female with a haematocrit value of
25%. Correct her reticulocyte count. Show calculation.

Answer 1

Q2. Calculate the relative reticulocyte count?

1 st slide = 15 reticulocytes counted in 1000 cells

Reticulocyte count in percentage = 15 reticulocytes counted in 1000 cells = 15/1000 cells.

Divide both sides by 10,

1.5/100 cells.

Reticulocyte count in percentage is 1.5%.

2nd slide = 17 reticulocytes counted in 1000 cells =

17 reticulocytes counted in 1000 cells = 17/1000 cells.

Divide both sides by 10,

1.7/100 cells.

Reticulocyte count in percentage is 1.7%

Q3. Corrected reticulocyte value if the subject is an adult female with . a haematocrit value of 25%

Corrected reticulocyte count = reticulocyte % × (subject's Hct/normal Hct).

Normal hematocrit value for female= 37% to 48%

Subject's hematocrit = 25%

By applying, 1.5 * (25/37) to 1.5 * (25/48)

(25/37)*1.5 = 0.6756 * 1.5 = 1.01

(25/48)*1.5= 0.528 *1.5 = 0.78

For slide 1, corrected value range between 0.78 - 1.01.

Similarly for slide 2,

1.7 * (25/37) to 1.7 * (25/48)

(25/37)*1.7 = 0.6756 * 1.7 = 1.15

(25/48)*1.7= 0.528 *1.7 = 0.89

For slide 1, corrected value range between 0.89 - 1.15.