Question

In: Chemistry

Suggest some possibilities as to why your buffer pH might not be exactly 8.0

Suggest some possibilities as to why your buffer pH might not be exactly 8.0

Solutions

Expert Solution

    Buffer solution is a aqueous solution consisting of a mixture of a weak acid and its conjugate base or vice versa.

    conjugate base is a species formed from an acid after donating a proton to base.

   Example :      HO-​ +  H​3NH​+ ​    <---->    H2​O              +        NH3      

                       Base      Acid                   Conjugate acid     conjugate base

​   Buffer solutions are used as means of keeping pH at a nearly constant value when a strong alkali or acid is added to it.


   pH of Buffer need not be exactly 8.0.   There are different buffer solution which are available or can be created to be used for mainting pH constantly at any pH value ranging from 3 - 8.

usually Buffer can be classified as acidic, alkaline or nuetral buffers, depending on the pH of the buffer.Acidic buffer will have pH less than 7 , Basic buffer will have pH more than 7 and nuetral buffer will have pH around 7.


Some examples of acids which with thier conjugate base can be used to prepare acidic buffer   

Acidic buffer

pH Range
citric acid buffer 2-7
acetic acid buffer 3.8-5.8

Some examples of base which with thier conjugate acid can be used to prepare basic buffer   

Alkaline buffer pH range
      Borate 8.2-10.2
CHES 8.3-10.3​

  Citric acid in combination with Disodium Hydrogen Phosphate can be used to prepare Buffer solution of wide range of pH ranging from 2-8 by just varying the concentrations of the Citric acid and Disodium Hydrogen Phosphate in the mixture. This buffer is called universal buffer or Mclvaine's buffer


pH range of buffer can be taken to pH of wide range by mixing two buffer.


Related Solutions

Describe the preparation of 1L of a 0.1 M phosphate buffer at pH 8.0. The pKa...
Describe the preparation of 1L of a 0.1 M phosphate buffer at pH 8.0. The pKa for the buffer is 6.86
Suppose you want to make 500 mL of a 0.20M Tris buffer at pH 8.0. On...
Suppose you want to make 500 mL of a 0.20M Tris buffer at pH 8.0. On the shelf in lab, you spot a bottle of 1.0M Tris at pH 6.8 and realize you can start with that to make his new buffer. Assuming you have 5.0M HCl and 5.0M NaOH at your disposal, how could you make this 0.20M Tris buffer at pH 8.0 from the 1.0M Tris, pH 6.8? (The pKa of Tris is 8.06.)
1. Tris buffer a. 100 mM Tris-HCl, pH 8.0 b. 100 mM Tris-HCl, pH 6.0 Make...
1. Tris buffer a. 100 mM Tris-HCl, pH 8.0 b. 100 mM Tris-HCl, pH 6.0 Make this buffer by dissolving an appropriate amount (which you must calculate using the formula weight) of solid Tris in APPROXIMATELY 80 mL of water. Use the Henderson-Hasselbalch equation to calculate how much 1 M HCl is needed to achieve the final pH?
Materials: Reaction Buffer 0.5 M Tris HCl pH 8.0 with 5 mM MgCl2 Alkaline phosphatase: 500...
Materials: Reaction Buffer 0.5 M Tris HCl pH 8.0 with 5 mM MgCl2 Alkaline phosphatase: 500 μg/mL stock solution in the buffer above. Keep enzyme on ice! p-nitrophenyl phosphate: 1000 μM and 10000 μM stock solutions in reaction buffer 1) Using the enzyme stock solution above and the dilution equation, calculate the volumes needed to prepare 2 mL of three enzyme dilutions consisting of 100 μg/mL, 125 μg/mL, and 150 μg/mL. Use the reaction buffer to make these dilutions. 2)...
Buffer pH by Design Given that Kb = 5.6×10-4 for ethylamine: To prepare exactly 450 mL...
Buffer pH by Design Given that Kb = 5.6×10-4 for ethylamine: To prepare exactly 450 mL of a buffer solution which has a pH of 10.89 and a final ethylammonium chloride salt concentration [CH3CH2NH3+] = 0.120 M. What mass of CH3CH2NH3Cl(s), would be needed and volume of 3.00 M sodium hydroxide solution needed plus enough water to make up the total volume.
Buffer pH by Design Given that Kb = 5.6×10-4 for ethylamine: To prepare exactly 450 mL...
Buffer pH by Design Given that Kb = 5.6×10-4 for ethylamine: To prepare exactly 450 mL of a buffer solution which has a pH of 10.89 and a final ethylammonium chloride salt concentration [CH3CH2NH3+] = 0.120 M. What mass of CH3CH2NH3Cl(s), would be needed and volume of 3.00 M sodium hydroxide solution needed plus enough water to make up the total volume.
Buffer pH by Design Given that Kb = 5.6×10-4 for ethylamine: To prepare exactly 450 mL...
Buffer pH by Design Given that Kb = 5.6×10-4 for ethylamine: To prepare exactly 450 mL of a buffer solution which has a pH of 10.89 and a final ethylammonium chloride salt concentration [CH3CH2NH3+] = 0.120 M. What mass of CH3CH2NH3Cl(s), would be needed and volume of 3.00 M sodium hydroxide solution needed plus enough water to make up the total volume.
Buffer pH by Design Given that Kb = 1.8×10-5 for ammonia: To prepare exactly 650 mL...
Buffer pH by Design Given that Kb = 1.8×10-5 for ammonia: To prepare exactly 650 mL of a buffer solution which has a pH of 9.47 and a final ammonium chloride concentration of 0.140 M, one would need to use what (mass) of ammonium chloride, and what (volume) of 2.50 M sodium hydroxide solution, plus enough water to make up the total volume.
Suggest and explain some actions that the government might take to increase the level of efficiency...
Suggest and explain some actions that the government might take to increase the level of efficiency in the market for education?
Bioinformatics: What could be some of the reasons why your query sequence did not exactly match...
Bioinformatics: What could be some of the reasons why your query sequence did not exactly match the sequence in the database (think of sampling, sequencing, and biological reasons).
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT