Question

Describe, in detail, how you would obtain a gene from a tomato plant and express this protein in bacterial cells. BamHI sites at either end surround the tomato gene of interest. You may use any vector of your own design in the process.

Answer 1

First the tomato cells are homogenized and the DNA is isolated. The isolated DNA is then fragmented using BamH1 resteiction endonuclease. We might obtain many genes but our gene of interest is selected using specific protein assay. The selected fragment is then transfered to a vector say E. coli plasmid by using same BanH1 restriction enudonuclease. The BamH1 will cut the vector plasmid and our gene is inserted and ligated with ligase enzyme. Now the plasmid in incooperated with our gene of interest . When the bacteria express its genes, our gene of interest also will expressed. So, we can easily obtain our desired protein from bacteria.