Question

In: Biology

Describe, in detail, how you would obtain a gene from a tomato plant and express this...

Describe, in detail, how you would obtain a gene from a tomato plant and express this protein in bacterial cells. BamHI sites at either end surround the tomato gene of interest. You may use any vector of your own design in the process.

Solutions

Expert Solution

First the tomato cells are homogenized and the DNA is isolated. The isolated DNA is then fragmented using BamH1 resteiction endonuclease. We might obtain many genes but our gene of interest is selected using specific protein assay. The selected fragment is then transfered to a vector say E. coli plasmid by using same BanH1 restriction enudonuclease. The BamH1 will cut the vector plasmid and our gene is inserted and ligated with ligase enzyme. Now the plasmid in incooperated with our gene of interest . When the bacteria express its genes, our gene of interest also will expressed. So, we can easily obtain our desired protein from bacteria.

gladiator answered 2 years ago
Next > < Previous

Related Solutions

​​​​​​​Describe how you can use PCR to obtain a gene fragment X that can be cloned...
​​​​​​​Describe how you can use PCR to obtain a gene fragment X that can be cloned into a plasmid vector for expression of the recombinant protein. Include in your description how the PCR reaction will be performed, how the amplicon will be analyzed, the use of restriction enzymes, how cloning will be achieved, how you will know that you have a recombinant containing the insert gene fragment X and how you will detect the recombinant protein.
Describe how you can use PCR to obtain a gene fragment X that can be cloned...
Describe how you can use PCR to obtain a gene fragment X that can be cloned into a plasmid vector for expression of the recombinant protein. Include in your description how the PCR reaction will be performed, how the amplicon will be analyzed, the use of restriction enzymes, how cloning will be achieved, how you will know that you have a recombinant containing the insert gene fragment X and how you will detect the recombinant protein.
a) Please describe in detail what PROFITABILITY RATIO attempt to measure? b) Where would you obtain...
a) Please describe in detail what PROFITABILITY RATIO attempt to measure? b) Where would you obtain such information? c) For what period of time and what other comparisons would assist your analysis. d) What management policies would have a positive affect on this analysis tool? Please do part B-D
1. Describe in detail how a protein-encoding gene in a eukaryote is transcribed as mRNA, and...
1. Describe in detail how a protein-encoding gene in a eukaryote is transcribed as mRNA, and what events happen to the mRNA before it can be translated into a protein. 2.You want to investigate the effect of a probiotic on your gut microbiome- the population of bacteria living in your digestive tract. You collect faecal samples prior to and after consumption of the probiotic. Describe in detail how you would sequence the metagenome of the bacteria in these faecal samples...
1. Describe in detail how a protein-encoding gene in a eukaryote is transcribed as mRNA, and...
1. Describe in detail how a protein-encoding gene in a eukaryote is transcribed as mRNA, and what events happen to the mRNA before it can be translated into a protein. 2.Describe the principles behind and the applications of the following: a) Northern blotting b) Site-directed mutagenesis c) DNase l footprinting d) Fusion protein vectors e) Sanger Sequencing of DNA 3.Describe six differences between DNA replication in bacteria compared with eukaryotes.
how do you make sure you obtain vectors with the gene of interest and not just...
how do you make sure you obtain vectors with the gene of interest and not just some random fragment of DNA that a restriction enzyme also cut out.(both the GOI and the random fragment have the same ends)
Describe in detail the German culture as it relates to beer consumption and how you would...
Describe in detail the German culture as it relates to beer consumption and how you would market coors beer in Germany.
Describe in detail the German culture as it relates to been consumption and how you would...
Describe in detail the German culture as it relates to been consumption and how you would market coors beer in Germany.
Describe human mitosis in detail. How is it different than plant meiosis? In this article, how...
Describe human mitosis in detail. How is it different than plant meiosis? In this article, how were the organism knockdowns and knockouts produced?
You have identified a mutation in a gene that results in enhanced drought resistance in tomato....
You have identified a mutation in a gene that results in enhanced drought resistance in tomato. It appears to encode a transporter. What will you have to do to determine whether the protein is associated with passive, active, or secondary active transport?
ADVERTISEMENT
Subjects
ADVERTISEMENT
Latest Questions
ADVERTISEMENT