1. Which organism is easier to grow in the laboratory, E. coli, or Neisseriae gonorrhea? 2....

1. Which organism is easier to grow in the laboratory, E. coli, or Neisseriae gonorrhea?

2. Describe the growth of E. coli in (on) soft agar.

Expert Solution

E.coli grow in digestive tract of warm blooded animals in large number. E.coli grow fast and easy in the laboratory as it can grow best at temperature 37.4^oC which is easy to maintain in laboratory.It can obtain nutrients from variety of sources as it mainly consume digested food in stomach.Grow aerobically and anaerobically. Grow fast and cell double every 20 minutes.Most are harmless strains eventhough exceptions are there. Neisseriae are very sensitive, specific and pathogenic. Bacterial viability may loss in transport, negative results may occur due to sampling errors. Modification of common laboratory technique are required. CO₂ is required for growth and glucose depletion in the media leads to autolysis of bacteria and cell death.

Soft agar is used to determine chemotaxis genotype.Chemotaxis is the directed movement towards environmental condition.Soft agar concentration supress the thermal and biological convecation in liquid media and able to detect the microrganisms ability to form colonies and motilty.E.coli undergoes swimming and swarming in the media. E.Coli are spread to nutrient buffer by capillary and are found in bands by depleting oxygen and nutrients in the media.Sharp circular rings formed outward from the colonies . Non chemotactic microorganisms grow uniformly. In E Coli chemotaxis with runs and tumbles can be observed. Runs towards attractants and very few tumble, that is swimming away from the attractant and change direction.

gladiator answered 1 year ago

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