Question

2. Protein-DNA binding

a) List the types of interactions that are involved between a protein that binds DNA non-specifically and DNA.

b) List two proteins that bind to DNA non-specifically.

c) List the types of interactions usually involved between a sequence-specific DNA binding protein and DNA.

d) List two proteins that bind DNA specifically.

e) What is the difference between non-specific and specific protein binding to DNA?

Answer 1

ANS a) There are two types of interactions that are involved between a protein that binds DNA non-specifically and DNA.DNA has Thymine, where as RNA has Uracil. RNA nucleotides include sugar ribose, rather than the Deoxyribose that is part of DNA. Functionally, DNA maintains the protein-encoding information, whereas RNA uses the information to enable the cell to synthesize the particular protein.

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ANS b) DNA motifs and histones that form part of the structure of DNA and bind to it non specifically.

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ANS c) Among the proteins that bind to dna are transcription factors that spark off or repress gene expression by binding to dna motifs and histones that shape part of the shape of dna and bind to it less especially.dna has thymine, in which as rna has uracil. rna nucleotides include sugar ribose, instead of the deoxyribose that is part of dna. functionally, dna maintains the protein-encoding statistics, whereas rna uses the information to permit the cell to synthesize the specific proteindna-binding proteins include transcription elements which modulate the process of transcription, numerous polymerases, nucleases which cleave dna molecules, and histones which can be worried in chromosome packaging and transcription inside the cell nucleus

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ANS d) DNA binding proteins are proteins composed of dna-binding domain names and therefore have a selected or widespread affinity for both single or double stranded dna dna-binding proteins encompass transcription factors which modulate the procedure of transcription, numerous polymerases, nucleases which cleave dna molecules, and histones which are involved in chromosome packaging and transcription within the cell nucleus. dna-binding proteins can incorporate such domains because the zinc finger, the helix-turn-helix, and the leucine zipper (amongst many others) that facilitate binding to nucleic acid. there are also more unusual examples which includes transcription activator like effectors

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ANS e) Among the proteins that bind to dna are transcription factors that spark off or repress gene expression by binding to dna motifs and histones that shape part of the shape of dna and bind to it less especially.dna has thymine, in which as rna has uracil. rna nucleotides include sugar ribose, instead of the deoxyribose that is part of dna. functionally, dna maintains the protein-encoding statistics, whereas rna uses the information to permit the cell to synthesize the specific proteindna-binding proteins include transcription elements which modulate the process of transcription, numerous polymerases, nucleases which cleave dna molecules, and histones which can be worried in chromosome packaging and transcription inside the cell nucleus