Question

4. What does enzymatic kinetics imply and how can we classify it? (Bibliography)

Answer 1

Enzyme kinetics is basically the study of reactions that are catalysed by enzymes. As the name suggest, enzyme kinetics deals with the reaction rate of the chemical reaction and also deals with various factors that can directly or indirectly affect the rate of reaction. For example,

E + S ? ES ? ES* ? EP ? E + P is the reaction with intial substrate S and Enzyme E, and final products Enzyme E and Product P. But this reaction also involves various intermediate steps which are important for the study of reaction kinetics. Enzyme kinetics helps to understand catalytic mechanism of the enzyme and its role in metabolism. Enzyme kinetics also deals with inhibition of reactions. There are many compounds which can inhibit a reaction and they are classified as reversible inhibition (further classified into competitive inhibition and non competitive inhibition) and irreversible inhibition. The reaction rate of an enzymatic reaction can be calculated by Michaelis Menten equation which is as follows,

Enzymes are classified on the basis of

1. The substrate acted upon

2. The reaction catalysed

Enzyme Kinetics study vary from reaction to reaction. The reaction can be

1. Single substrate reaction : A ---> B

2. Multiple substrate reaction : C + D ---> E + F

Single substrate reactions are rare unlike multiple substrate reactions.

Bibliography :

Campbell and Farell, Biochemistry, 5th edition

Price and Stevens, Fundamentals of Enzymology, Oxford Univ. press, 3^rd Ed

wikipedia