if LIA tubes were accidentally prepared with no glucose how the results of the test ne...

if LIA tubes were accidentally prepared with no glucose how the results of the test ne changed

Expert Solution

Lysine iron agar (LIA) tubes are used to test the ability of the microbes to deaminate or decarboxylate lysine. Lysine deamination is an aerobic process, therefore occurs on the slant of the media. Lysine decarboxylation is an anaerobic process and so occurs in the butt of the media. Tubes contain lysine, peptones, a small amount of glucose, ferric ammonium citrate, and sodium thiosulfate.

Principle behind this process is:

Acidic cindition indicated by yellow when glucose is fermented in butt of the slant.
In presence of lysine decarboxylase, acidic environment is neutralized and reverts to the alkaline state indicated by purple coloration.
WHEN THERE IS NO GLUCOSE, THE BUTT OF THE TUBE REMAINS PURPLE ALKALINE WHEN THE ORGANISM SECRETE LYSINE DECARBOXYLASE OR NEUTRAL WHEN THE ORGANISM IS UNABLE TO PRODUCE LYSINE DECARBOXYLASE .
When oxidative deamination of lysine occurs, it forms burgundy color on the slant and in absence of oxidative deamination, purple remains

Bromocresol purple, the pH indicator, is yellow at or below pH 5.2 and purple at or above pH 6.8.

gladiator answered 1 year ago

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