Fill in the blank: When performing a negative stain, the ____________ charged proteins inside the bacterial...

Fill in the blank:

When performing a negative stain, the ____________ charged proteins inside the bacterial cell will be repelled by the _____________ charged dye, thus allowing for visualization under the microscope.

When performing a simple bacterial stain, the _______________ charged proteins inside the bacterial cell will be attracted to the ____________ charged dye, thus staining the interior of the cell to allow for visualization under the microscope.

Expert Solution

Ans:

When performing a negative stain, the ______negative______ charged proteins inside the bacterial cell will be repelled by the _______negative______ charged dye, thus allowing for visualization under the microscope.

When performing a simple bacterial stain, the ________negative_______ charged proteins inside the bacterial cell will be attracted to the _______positive_____ charged dye, thus staining the interior of the cell to allow for visualization under the microscope.

Explanation: In negative staining, a negatively charged dye like nigrocin (acidic) is repelled by the negatively charged protein of bacterial cell wall. So, this stain can not penetrate the bacterial cell and its stain only background, not the cell.

In simple bacterial staining or positive staining, a positively charged dye (basic) is attracted by the negatively charged protein of bacterial cell wall. So, this stain penetrates the bacterial cell and stain its.

gladiator answered 1 year ago

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