Question

Duplication of a genome once per cell cycle is, in essence, a biosynthetic process and, hence, demands the input of energy. Moreover, successful semi-conservative replication of the genomic DNA requires the action of many different components. Based on your understanding of the information and general principles covered in lecture, fill in the spaces provided with your responses to the questions asked about the ten proteins below.

Protein	Nucleotide required? (Yes or No)	If Yes, which nucleotide?
Cdt1
DnaA
DnaB
DnaC
DNA ligase I
E.coli Pol I
Mcm7
Orc5
Psf1
Rfc5

Answer 1

1. Cdt1 - is the protein responsible for performing the replication process. No nucleotides are required for DNA replication. It reacts with another protein for replication.

2. Dna A - is responsible for separation of DNA strands and nucleotides are not involved.

3. DNA B - helps in the process of replication and nucleotides are not present.

4. DNA C - is the loading factor of dnaB and requires no nucleotides.

5. DNA ligase 1 - helps in joining mechanism of DNA strands. It requires ATP and helps to join the nucleotides in two strands.

6. Ecoli Pol I - Used in replication. Nucleotides are required and fills in nucleotides according to okazaki fragments with A,G,C,U.

7. Mcm 7 - DNA replication licensing factor. Nucleotides absent

8. Orc5 - present in dna binding complex. Nucleotides absent

9. Psf 1 - DNA replication factor. Nucleotides absent

10. Rfc 5 - protein complex required for DNA `replication. Nucleotides absent.