which isolation technique will be lest suitable to the isolation of salmonella typhi? streak plate, pour...

which isolation technique will be lest suitable to the isolation of salmonella typhi? streak plate, pour plage or spread plate

Solutions

Expert Solution

Answer - Pour plage.
Explanation - Most clinical and environmental specimens contain several different microorganisms. To obtain a pure culture, individual organisms must be isolated.
The most common method of isolation is the streak plate , in which a sterile loop is inserted into a sample and streaked onto a plate in a pattern, to obtain individual colonies.

Spreading - This technique typically is used to separate microorganisms contained within a small sample volume, which is spread over the surface of an agar plate, resulting in the formation of discrete colonies distributed evenly across the agar surface when the appropriate concentration of cells is plated.

In the case of pour plage - use of relatively hot agar carries the risk of killing some sensitive contaminants. Colonies of different species within the agar appear similar — so it is difficult to detect contaminants.The reduced growth rate of obligate aerobes in the depth of the agar. Preparation for the pour plate method is time consuming compared with streak plate/and or spread plate technique

gladiator answered 1 year ago

Next > < Previous

Related Solutions

how would loop dilution, spread plate, and streak plate isolation techniques be affective by the use...

how would loop dilution, spread plate, and streak plate isolation techniques be affective by the use of selective medium

What is the main advantage and disadvantage a pour plate has over a spread plate in...

What is the main advantage and disadvantage a pour plate has over a spread plate in counting bacteria?

Streak Plate Drawing or Picture of Plate Description of colonies/plate: Gram Stain Draw and color Gram...

Streak Plate Drawing or Picture of Plate Description of colonies/plate: Gram Stain Draw and color Gram reaction, size, shape/arrangement: (what you see under microscope) Identification of: Serratia marcescens 1)STREAK PLATE OF Serratia marcescens 2)RESULTS OF STREAK PLATE: A: Explain the Gram reaction, size, shape, and arrangement you would expect to see after performing these procedures of your bacteria. *Draw what your streak plate would look like on your worksheet. B: What would a successful streak look like? What could be...

If you were using the quadrant streak plate method to plate a very dilute broth culture...

If you were using the quadrant streak plate method to plate a very dilute broth culture (with many fewer bacteria than the broth used for the plate pictured here), would you expect to see single, isolated colonies in quadrant 4 or quadrant 3? Explain your answer.

what is streak plate inoculation techniques what is importance of colony calculating forming unit and bacteria...

what is streak plate inoculation techniques what is importance of colony calculating forming unit and bacteria differentiation what is Grams staining what is gram positive and negative bacteria

A few days after you have completed the streak plate method, you observe not one single...

A few days after you have completed the streak plate method, you observe not one single isolated colony on the TSA plate. Explain why this may have happened. How does dental plaque form? How does tooth decay occur?

Question 1: Why a pure colony isolation on the plate is necessary? (2 marks) . Question...

Question 1: Why a pure colony isolation on the plate is necessary? . Question 2: Briefly define the use of the following media in combination with primary culture media for isolation of organisms from clinical specimens. Support your answer by quoting examples for each category: (1.5 x 4 = 6 Marks) 1. An enrichment broth 2. Nutritional agar 3. Selective agar 4. Differential media . Question 3: What are the limitations associated with placing too much organism on the slide...

Question 1: Why a pure colony isolation on the plate is necessary? (2 marks) Question 2:...

Question 1: Why a pure colony isolation on the plate is necessary? Question 2: Briefly define the use of the following media in combination with primary culture media for isolation of organisms from clinical specimens. Support your answer by quoting examples for each category: (1.5 x 4 = 6 Marks) 1. An enrichment broth 2. Nutritional agar 3. Selective agar 4. Differential media Question 3: What are the limitations associated with placing too much organism on the slide prior to...

Which of the following is an example of mechanical isolation?

Which of the following is an example of mechanical isolation?Many insects have intricate "lock and key" mechanisms that prevent the male genitalia of one species from engaging with the female genitalia of a different species.A sponge excretes eggs and sperm into the water, but only gametes from the same species will fertilize each other.A mule, the offspring of a horse and a donkey, is sterile.A female moth produces a pheromone to which male moths of another species are indifferent.

Describe the steps of aseptic technique when transferring culture to a plate. Describe the steps of...

Describe the steps of aseptic technique when transferring culture to a plate. Describe the steps of streaking for isolation. What is the goal of streaking a plate this way? Please post with typed responses - no handwritten or picture responses.

Subjects