METHODOLOGY WITH IMAGE
WHAT IS THE PROCEDURE OF STEAK PLATE INOCULATION TECHNIQUES
HOW TO CALCULATE COLONY
HOW TO APPLY GRAMS STAINING
DISCUSS ABOUT BACTERIA DIFFERENTIATION TECHNIQUES
IMPORTANT POINT OF LITERATURE METHODOLOGY AND DISCUSSION
PARTS
Use R studio.
Bacteria in water are counted as colony-forming units (CFU’s)
per milliliter. Ten bottles of water are randomly
selected for sampling, with the intention of testing if two
different labs produce the same results. Each bottle is divided
into two parts, and then given to each of the two labs:
Bottle
1
2
3
4
5
6
7
8
9
10
Lab 1
875
959
475
589
925
1100
971
450
892
728
Lab 2
910
878
410
495...
why
is it important to study single colonies of bacteria?
what is a colony and how does it relate to a bacterial
cell?
what are the advantages of growing bacteria on a plate vs
broth?
how can you tell whether the colonies on your plate are from
the original culture and not contamiinants?
what is the bacteria of that is gram negative, acid fast
negative, and endospore forming? there is growth on blood and
macconkey not MSA.
options:
1, salmonella pulloram
2, enterobacter aerogenes
3, strep. mutans
4, micrococcus luteus
5, mycobacterium smegmatis
6, bacillus subtilis
7, staph. aureus
its
actually non-endosporing
What are the next steps once you have an isolated colony to
determine the bacteria growing in that colony? (These are some of
the I’s – selective media what might you suggest?)
What are the “firmicutes” (give examples with
importance)? What bacteria are found in the α (alpha), β (beta), γ
(gamma) Proteobacteria groups, indicating their
importance.
what is the importance of identifying unknown bacteria
in general? paragraph 1
describe the general strategy involved in identifying unknown
strains? paragraph 2