Question

7. Suppose that you have just graduated from college and have started working at a biotechnology firm. Your first job assignment is to clone the pig gene for the hormone prolactin. Assume that the pig gene for prolactin has not yet been isolated, sequenced, or mapped; however, the mouse gene for prolactin has been cloned, and the amino acid sequence of mouse prolactin is known. Briefly explain two different strategies that you might use to find and clone the pig gene for prolactin.

8. Describe how the genome of an organism of your choice has been successfully engineered to improve a trait of economic importance in the organism.

Answer 1

We can use two stratagy- 1st at gene level and 2nd at protein level. At gene level, we can use gene sequence of prolactin from mouse gene and do ncbi blast with whole genome of pig and analysed the result. In this result, data will show that where are the gene of prolactin located on pig genome and how much similarities it show with nucleotide of mouse gene.

Similarly we can do with amino acid sequence, we can take protein sequence of prolactin from mouse prolactin and used ncbi blast and see the similar sequence present in pig organism. Or we can also annotate the amino acid sequence using nucleotide sequence obtained in above ncbi blast data. And match this amino acid sequence with mouse prolactin sequence.

We can increase the yield of prolactin by using strong promotor and enhancer region so that prolactin transcription rate can be increased and more yield of prolactin can be done and for this we can do certain changes in gene sequence of prolactin.

Hope it's clear...thanjs