In: Statistics and Probability
6. a. If the
oligopeptide AFVRWGTHLESKLVMSLDIHLRF were cleaved completely by
reaction with trypsin and V8 protease...
6. a. If the
oligopeptide AFVRWGTHLESKLVMSLDIHLRF were cleaved completely by
reaction with trypsin and V8 protease and the products subjected to
ESI TOF-MS in positive ion mode with the peptides in a pH 2
solution, which product will reach the detector last? Explain
why.
- If the Lys on the oligopeptide were to be acetylated on its
side chain, would the last peptide to reach the detector be
changed? Explain.
- To determine the Mr of a purified protein, you
subject it to ESI TOF-MS in positive ion mode with the protein in a
pH 2 solution. The spectrum shows a series of peaks with the two
most prominent neighboring peaks having m/z ratios of 648.78 and
659.52. What is the uncharged mass of the protein (i.e.,
Mr at its pI) in the 648.78 peak?
- Is the charge on the protein in part c consistent with the
protein having a typical basic amino acid composition? Justify your
conclusion mathematically.