Question

In: Biology

Using the principles of second generation sequencing technology we learned in class, describe in a step...

Using the principles of second generation sequencing technology we learned in class, describe in a step by step fashion how targeted sequencing of DNA and RNA were performed in this study? Write your response in paragraph form.

In general, be able to explain in a step by step fashion the workflows involved in carrying out sequencing with each of the different sequencing platforms utilized in these studies. Know what each step accomplishes. Write your response in paragraph form.

What are the advantages and disadvantages of each kind of platform (2nd, 3rd, 4th) generation)?

Solutions

Expert Solution

Answer to first para:

Principle of second generation sequencing is similar to Sanger sequencing. To prepare the targeted nucleic acid (DNA/RNA) THE FOLLOWING STEPS ARE FOLLOWED.

1.Genomic DNA is isolated and randomly broken into fragments.

2.Template is created and amplified by Polymerase chain reaction

3.Single stranded template is immobilised on a solid support

4.The primer(RNA) molecule is covalently attached to the solid support and it is hybridised with template.DNA polymerase is made to bind to the immobilised primed template to initiate the second generation sequencing reaction.

5. It results in population of identical templates.Each one is taken for sequencing reaction.They aligned to a known reference sequence and data is analysed.

Answer to 3rd Para:

Advantages:

Second generation is most commomnly used,substancially cheaper. It is faster and helps in massive sequencing data.It is most commonly used.

In Third generation nucleici acids are sequenced directly and have higher output.

In fourthgeneration sequencing genomic analysis is directly in the cell(in vivo) and it is the most recent.

gladiator answered 1 year ago
Next > < Previous

Related Solutions

Illumina sequencing is a ‘Next Generation Sequencing’ technology. Explain the flow of how the Illumina sequencing...
Illumina sequencing is a ‘Next Generation Sequencing’ technology. Explain the flow of how the Illumina sequencing technique workk. (1.5 pts) There are several commercially available DNA polymerases that are used in sequencing. Two of these DNA polymerases are Taq and Q5. Explain some of the similarities and differences between these two polymerases. (1 pt)
Why was the sequencing technology of next-generation a breakthrough relative to classical gene-by-gene sequencing technology?
Why was the sequencing technology of next-generation a breakthrough relative to classical gene-by-gene sequencing technology?
what is the history of the next-generation sequencing in DNA technology and tools?
what is the history of the next-generation sequencing in DNA technology and tools?
What are the history and steps of the next-generation sequencing in DNA tools and technology?
What are the history and steps of the next-generation sequencing in DNA tools and technology?
Through Next-Generation Sequencing, we have learned a lot about the human gut microbiome, including community demographics,...
Through Next-Generation Sequencing, we have learned a lot about the human gut microbiome, including community demographics, establishment of the microbiome and microbiome stability. Design a study to determine the effects of daily kombucha consumption on gut microbiome composition and function. Be sure to describe the model system, controls, types of data to be collected, methods of data analysis, and limitations of the study. Also indicate whether the data you gather will provide correlative or causative evidence of the effects of...
Next-generation sequencing technology has revolutionized nearly every biological field of study. A variety of techniques are...
Next-generation sequencing technology has revolutionized nearly every biological field of study. A variety of techniques are used to create a genomic library prior to sequencing. However, cost is still a factor when deciding on which method to use for a particular research question. (A) Briefly describe the technique of restriction-site associated DNA sequencing (RADseq). What is the difference between single-end and paired-end sequencing? (B) Suppose you were interested in examining the population genomic structure of NYC rats throughout the five...
Next-generation sequencing technology has revolutionized nearly every biological field of study. A variety of techniques are...
Next-generation sequencing technology has revolutionized nearly every biological field of study. A variety of techniques are used to create a genomic library prior to sequencing. However, cost is still a factor when deciding on which method to use for a particular research question. (A) Briefly describe the technique of restriction-site associated DNA sequencing (RADseq). What is the difference between single-end and paired-end sequencing? (B) Suppose you were interested in examining the population genomic structure of NYC rats throughout the five...
Next Generation Sequencing (NGS) technology has revolutionize genomics, transcriptomics and other aspects of biomedical and non-biomedical...
Next Generation Sequencing (NGS) technology has revolutionize genomics, transcriptomics and other aspects of biomedical and non-biomedical research. Discuss and provide evidence to defend this statement
What are some generic names of meds in this class – first and second generation? What...
What are some generic names of meds in this class – first and second generation? What is the side effects of traditional first generation antihistamines? What precautions would the nurse need to consider? What generation of antihistamines are non-sedating with fewer anticholinergic effects?
A researcher wants to clone genes using recombinant gene technology as part of a genome sequencing...
A researcher wants to clone genes using recombinant gene technology as part of a genome sequencing project. There are eight steps below. First, cross out the two steps that are not part of this process. Then, put the remaining six steps in the correct order. A Insert recombinant plasmid into bacterium. B Cut the genome into fragments using restriction enzymes. C Determine activity using laser. D Use DNA Ligase. E Make cDNA using reverse transcriptase. F Grow bacteria and add...
ADVERTISEMENT
Subjects
ADVERTISEMENT
Latest Questions
ADVERTISEMENT