Question

what is the history of the next-generation sequencing in DNA technology and tools?

Answer 1

Next-generation sequencing (NGS) is a sequencing technology that uses DNA, RNA, or methylation sequencing.
Next or second-generation of sequencing has a large parallel analysis and high throughput results with reduced cost. Due to massively parallel sequencing, reading of large amount i.e 1-100 million of short, 50-400 bases, DNA fragments is achieved.

Sequencing systems:

i) Roche 454 System- It is a system that detects the pyrophosphate that is released during nucleotide incorporation in the sequence.

ii) AB sequencing: That is possible by Oligo Ligation Detection (SOLiD),

iii) Illumina GA/HiSequ System: Solexa’s Genome Analyzer (GA)

History of sequencing

Balasubramanian and Klenerman approached the capital firm Abingworth Management and obtained initial seed funding to make Solexa in 1998. The first Solexa sequencer, the Genome Analyzer, was launched in 2006 and gave scientists the power to sequence 1 gigabase (Gb) of knowledge during one run.

Solexa was acquired by Illumina in early 2007. Various microbe, plant, human, and animal genomes are sequenced using this technology.

These highly effective sequencing systems demonstrate their own advantages in terms of reading length, accuracy and price. Briefly, the Illumina HiSeq 2000 features the foremost important output and lowest reagent cost, the SOLiD system has the foremost effective accuracy and thus the Roche 454 system has the longest read length.