Imagine a mutated bacterial cell that never expresses the lac genes, even under the conditions where...

Imagine a mutated bacterial cell that never expresses the lac genes, even under the conditions where they would normally be expressed. Which of the following mutations would explain this phenotype/

The lac repressor is mutated so that it cannot bind to the operator
The operator is mutated so that it cannot bind to the lac repressor
The promoter is mutated to be more like the consensus sequence
CRP is mutated so that it cannot bind to the CRP response element

Expert Solution

c) The promoter is mutated to be more like the consensus sequence

the lac operon is inhibited by lac repressor by binding to the operator in the absence of an inducer, in the presence of an inducer lac repressor is inhibited so it cannot bind to the lac operator so the lac operon is expressed at higher levels.

so in

The lac repressor is mutated so that it cannot bind to the operator

and

The operator is mutated so that it cannot bind to the lac repressor

the lac operon has to expressed even in the absence of lactose.

The promoter is mutated to be more like the consensus sequence, then RNA polymerase cannot bind and transcribe the lac operon.

CRP is a protein that increases the transcription of lac operon when glucose is absent, if CRP is mutated, the lac operon cannot be expressed at higher levels in the absence of glucose.

gladiator answered 2 years ago

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