Question

1.

Which of the following events does not occur in PCR?    

Group of answer choices

A the DNA is denatured by high temperature

B the amount of DNA would double during each cycle, resulting in 8 times the original amount of DNA after 3 cycles

C Taq DNA polymerase and deoxyribonucleotides are used to elongate the primer strand of DNA

D the mixture is heated to allow primers to complementary base pair to the deoxyribonucleotides

2.

Which of the following is not a difference between DNA and RNA?

Group of answer choices

A in RNA, the sugar is deoxyribose, in DNA it is ribose

B No answer text provided.

C in RNA the base U is used in place of T in DNA

D in RNA, the sugar is ribose, instead of DNA it is deoxyribose

3.

a- (two words) ____ _____is the enzyme used in DNA fingerprinting

b- _____ blotting technique in used in DNA fingerprinting

c- Upon electrophoresis, DNA will migrate towards the ________ pole.

4.

In the dideoxy DNA sequencing method, the enzyme used is RNA polymerase.

Group of answer choices

True

False

5.

Meiosis occurs in one division and results in two daughter cells that are genetically identical.

Group of answer choices

True

False

6.

In a female, the inactivated Y chromosome is an example of heterochromatin.

Group of answer choices

True

False

7.

On a human chromosome, the long arm is called the "p arm" and the short arm is the "q arm."

Group of answer choices

True

False

Answer 1

1) PCR or the polymerase chain reaction is the technique that is used for producing the large number of the same gene or a piece of DNA . Taq polymerase is a Heat stable DNA dependent RNA polymerase . In each cycle, the gene replicatiom resulting in doubling the copy of gene and DNA structure is degaraded by high temperature

Hence option D is correct answer that does'nt occur in PCR

2) DNA is the deoxyribonucleotide Acid and RNA is the ribonucleotide Acid. DNA is made up of dexyribose sugar and RNA is made up of ribose sugar . In RNA , Uracil is present in place of thymine

Hence option A is correct answer
3) Restriction enzyme is used in the DNA fingerprinting . Restriction enzyme are of 2 types Endonuclease and the Exonuclease. Restriction enzyme are the DNA cutting enzymes that are found in certain Bacteria as a protection agent the bacteriophase. Restriction enzyme is used in biotechnology

Western blotting is used in the DNA fingerprinting there are mainly 5 steps in western blotting :- transfer, blocking , incubation :-primary and secondary , analysis DNA moves towards positive pole as it is negative in nature
4) False, It is also known as the Sanger sequencing is the technique for the sequencing of the section of DNA in the genome . It is done by the DNA dependent DNA polymerase enzyme .

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