Question

In a wet mount each of the following complications put false positives, false neg, no effect 1: over inoculate of the slide with organism for motile org, nonmotile org, 2. Cells attaching to the glass slide or cover glass more motile, nonmotile. 3. Receding water line motile organism, nonmotile, 4. Using an old culture motile, nonmotile

Answer 1

Over inoculation of the slide with motile organism will give false negative because there will not be enough room for organism to move and they will appear nonmotile.

Over inoculation of the slide with nonmotile organism will give false positive as motile organism will also appear nonmotile due to reasons mentioned above.

Cells attaching to the glass slide or cover slip with motile organism will give false negative because the organism that are stuck to the glass will not be moving.

Cells attaching to the glass slide or cover slip with nonmotile organism will give false positive as motile organism will also appear nonmotile due to reasons mentioned above.

Receding water line with motile organism will give false positive because the movement of water will give the impression as if cells are moving.

Receding water line with nonmotile organism will give false negative because the movement of water will make nonmotile organism appear motile.

Using an old culture with motile organism will give false negative because in old culture a motile organism would be dead in old culture and will appear nonmotile.

Using an old culture with nonmotile organism will give false positive because in old culture dead motile organism will also appear nonmotile.

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