What is the designation of the recipient cell after mating with an Hfr donor strain? 1....

What is the designation of the recipient cell after mating with an Hfr donor strain?

1. F-

2. F'

3. F+

D. Hfr

Solutions

Expert Solution

The transfer of the genetic material in the bacteria is possible by the help of conjugation. In conjugation a part of desired gene is transferred from donor bacteria to the receptient bacteria. It is a cell to cell contact between two bacterias which occurs through the donation of pilli( singular pillus). Let us take an example of conjugation in E-coli. There is an strain Hfr stain means desired gene (high frequency recombinant) is transferred or F- through the plasmid. Those cell which don't have plasmid will be called F-(recipient) and which have called F+.

The Hfr cell acts as a donor and F- acts as a recipient when Hfr transfer it's it's genome in F- through pilli it can not tranfer all of its DNA to the host cell because it lost the touch to the F- strain due to higher size and no homologous recombination occured which means entire genome is not tranferred. It entire genome gets transferred it will become Hfr.

Since not all the DNA is tranferred from HFr to F- the F- will remain remain as F-.

Hence the answer to this question is option 1 F-.

gladiator answered 1 year ago

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