Question

In: Biology

My bacteria is serratia marcescens and the test I am working on is nitrate reduction.

# Microbiology

My bacteria is serratia marcescens and the test I am working on is nitrate reduction. I believe I would get a positive reaction, but i'm not sure if it would turn red after adding in the reagents or after adding in the zinc?

Solutions

Expert Solution

Bacteria Serratia marcescens is short and the shape is rod in appearence. It's growth is possible during aerobic and anaerobic condition.Being Gram negative it has a cell wall ( thin)covered with outer membrane which consist of lipopolysacchrides. The outer membrane helps to uptake the nutrients.

Identification of Bacteria Serratia marcescens -:

On Nitrate Reduction: it gives positive results with nitrate as it accepts the final electron and not oxygen. Serratia marcescens generally gives red stain.

The bacteria is covered in broth of nitrate and after 4-5 hours if Nitrate is reduced to nitrite and nitrite itself forms nitrous acid. When this nitrous acid reacts with sulfanilic acid gives diazotized sulfanilic acid . It gives red colour on treatment with alpha napthylamine indicating positive results of Nitrate Reduction.

On adding zinc in positive Nitrate Reduction , the red colour will not appear but it gives red colour with other reagent indicating the positive results of Nitrate Reduction.

 


Related Solutions

I am working on extensive report for my Advanced Corp. Tax class and I am hoping...
I am working on extensive report for my Advanced Corp. Tax class and I am hoping to get a better understanding of the following: -In order for a corporation to make a valid “S” election, the corporation must be a “small business corporation.” Explain the meaning of “small business corporation” in this context. -Explain what ‘separately stated items’ are in regards to Partnerships and S corporations? Give an example of ‘separately stated items’ for each type of entity. -What requirements...
I am working on a project where I have to create my own organization (stem cell...
I am working on a project where I have to create my own organization (stem cell research) I need to go further in depth about the program or organization in terms of describing the program implementation and the project evaluation...
I am playing a game of Dungeon and Dragons with my family. My son’s character must...
I am playing a game of Dungeon and Dragons with my family. My son’s character must roll a critical hit, (19 or 20) on a 20 sided die. Without a critical hit, he cannot do enough damage to the troll. The troll will regenerate and kill me on the next turn. In addition to getting a high roll for hit chance, my son’s character must also get a high roll for damage. His weapon is a great sword and does...
My bacteria is staphylococcus epidermidis and these are the tests I need some help with:
# Microbiology  My bacteria is staphylococcus epidermidis and these are the tests I need some help with: PR-mannitol PR-raffinose I need to describe what results I would expect to get if I was completing this test in the lab.
I am working on a project that requires a measurement of acceleration. I have a ADXL345...
I am working on a project that requires a measurement of acceleration. I have a ADXL345 accelerometer, an aduino and a MAX7219 dot matrix display. I need help setting up a code for my aduino to display the acceleration from the ADXL345 accelerometer onto the MAX7219 display. Please help!
My name is Donna. I am a college student studying to be an English teacher. I...
My name is Donna. I am a college student studying to be an English teacher. I dislike inflation, my degree is costing me more than i had anticipated. Good thing that I am a senior, tuition when I started three years ago was 20% less. Maybe I should get my masters but that would put me unto more debt. Why do we have so much inflation? I work part time as a server and have a low fixed rate loan...
This is my lab and I am very confused regarding the calculations! It is supposes to...
This is my lab and I am very confused regarding the calculations! It is supposes to demonstrate Le Chatelier’s principle. After doing the following I need to calculate the molar absorbance for Co(H20)6 2+. The path length is 1cm and absorbance is .551. I got 5.51 L/mol *cm. I also need to calculate the CoCl4 2- molar absorbance which I do not understand. The absorbance is .570 and the path length is 1cm. I do not know how to find...
Suppose I am trying to guess the salary of one of my fellow professors who I...
Suppose I am trying to guess the salary of one of my fellow professors who I think is 40 years old, has been at xxuniversity for 5 years, and has 25 publications. I am provided with the following regression analysis results from several alternative models that predict salary (in CAD) from these variables, based on N=35 participants. Note: yrs = years. Model 1; R-squared = 0.578; Adjusted R-squared = 0.537 Coefficient Standard Error     t          p-value     Intercept 86248.19 10016.43   8.61  ...
I am considering selling my current home through an ownerfinanced arrangement. I have beenoffered...
I am considering selling my current home through an owner financed arrangement. I have beenoffered $1,000 per month for 20 years with the first payment due immediately plus anadditional $100,000 with the last payment at the end of 20 years. If the appropriate discountrate is 6% APR compounded monthly, then what is the equivalent cash offer for my house?
As an LPN working on a medical floor, I read the order for my patient that...
As an LPN working on a medical floor, I read the order for my patient that the physician had just written: “Obtain throat culture for C&S.” I knew that this meant the physician wanted a throat swab collected for culture and sensitivity testing. Culturing refers to identification of the bacteria causing the patient’s symptoms, while sensitivity will determine what antibiotic the bacteria identified will be sensitive to—in other words, which antibiotic has the ability to kill the bacteria. I gathered...
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT