Question

One of major problems is neuron cell dead and you are interested in neurodegenerative diseases. You are discovered molecule involved in cell survival. Could you please desing your experiments to answer below questions? a)how do you test this molecule protect neuron cell dead? b)if this molecule protect neuron cell dead, how do you test which molecule involved in neuron cell survive? c)how do show this molecule involved neuroprotection in vivo?

Answer 1

There are many neurodegenrative disease related to human being one of them is diabetic retinopathy ,in neurodegenrative disease many genes and metabolic pathways are involved here according to experiment molecules treated with neurodegenrative cell and detect the comparision between healthy neuron cell and degenrative cell if molecules works on neuron cell then there will be low degenerative value .

B this type of test is involved in epigenetic modification here a molecules deals with proteins and alter their work via enhancement or decreased .gives a specific molecules dose range and monitor their effect with time gives a idea about particular function.

C. Firstly it describe by in vitro experiment in which we extract a piece of neuron cell and gives a different dose of molecules in different time with their natural condition after that same treatment in vivo condition and if result is same than we can can say that yes this molecules is involved neuroprotection in vivo.