In: Biology
The purpose of subjecting your BSA protein standard plot data to the least squares statistical method was to
a. determine intuitively the best fit straight line.
b. statistically determine the best straight line through a series of experimental points.
c. determine the overall shape of the BSA standard curve.
d. determine the protein concentration in the unknown samples.
B - statistically determine the best line through a series of experimental points.
BSA standard plots are generally used to determine the concentration of protein sample.This is achieved by ploting responses of standards and determining the best fitting line between them. Using the absorbance values of unknown sample, the protein concetration is determined by the formula for the standard line.