What color do you expect a Gram-positive cell to be at each step of the Gram...

What color do you expect a Gram-positive cell to be at each step of the Gram staining procedure? Why?

What color do you expect a Gram-negative cell to be at each step of the Gram staining procedure? Why?

Expert Solution

	Colour of gram positive bacteria	Colour of gram negative bacteria
1st step, addition of crystal violet stain	Crystal Violet is the Purple coloured stain and therefore the bacteria turns purple	same as Gram Positive Bacteria
2nd step, addition of iodine	Crystal Violet iodine Complex are formed which are trapped in the mesh work of peptidoglycan layer and the bacteria appears purple	same as Gram Positive Bacteria
3rd step, addition of alcohol	Crystal Violet iodine complexes are not washed off from the cell because peptidoglycan is thick and the bacteria is still purple	Crystal Violet iodine complexes are washed off and bacteria appears Colourless
4th step, addition of safranine	Gram Positive Bacteria is still purple and will remain purple	Gram negative bacteria was colourless and now it will appear red

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gladiator answered 2 years ago

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