Describe the likely effect on gene expression of a mutation in the lac operon: a) that...

Describe the likely effect on gene expression of a mutation in the lac operon:
a) that delegate the operator
b) that mutates the lacI gene inactivating the repressor
c) that the promoter mutates in the -10 region

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gladiator answered 2 years ago

A cis-acting mutation that leads to MINIMAL expression of the lac operon would most likely be...

A cis-acting mutation that leads to MINIMAL expression of the lac operon would most likely be in the CAP binding site. Is this true or false, and why? Thanks!

Predict the level of gene expression of the lac operon, as well as the status of...

Predict the level of gene expression of the lac operon, as well as the status of the lac repressor and the CAP protein, when bacterial growth media contains the following sugars: a) no lactose or glucose, b) lactose but no glucose, c) glucose but no lactose, d) both lactose and glucose.

27. Be able to describe regulation of gene expression in prokaryotes (transcriptional, like the Lac Operon)...

27. Be able to describe regulation of gene expression in prokaryotes (transcriptional, like the Lac Operon) and in eukaryotes (transcriptional and epigenetic regulation).

A mutation prevents expression of the CAP protein. Will the Lac operon be expressed when lactose...

A mutation prevents expression of the CAP protein. Will the Lac operon be expressed when lactose levels are high and glucose levels are low? Explain your reasoning.

Predict the level of gene expression of the lac operon in the following situations: (WT- normal...

Predict the level of gene expression of the lac operon in the following situations: (WT- normal function, Mut- loss of function mutation) (please type so answer is easier to read) No lactose, WT repressor Lactose, WT repressor No lactose, Mut repressor Lactose, Mut repressor

What if you wanted to test the effect of sucrose containing media on lac operon expression?...

What if you wanted to test the effect of sucrose containing media on lac operon expression? Design an experiment that would allow you to accomplish this.

(a) Draw the lac operon. (b) What role does the lacI gene product play in expression of β-galacatosidase?

(a) Draw the lac operon.(b) What role does the lacI gene product play in expression of β-galacatosidase?(c) How does the lac repressor prevent binding of RNA polymerase?(d) What role does β-galactosidase play in regulation of the lac operon?(e) What is the purpose of the lacY gene product?

2. The -10 core promoter of the lac operon gene has the sequence TATGTT. Describe an...

2. The -10 core promoter of the lac operon gene has the sequence TATGTT. Describe an example of a base substitution mutation which would lead to increase transcription of the gene. Explain whether this mutation would increase or decrease the fitness of the organisms.

1. For the lac operon, explain what would happen and why regarding expression of the operon...

1. For the lac operon, explain what would happen and why regarding expression of the operon in each of the following scenarios. Be sure to include both positive and negative regulation in your explanation. a.) lactose is present, glucose is absent b.) lactose is absent, glucose is present c.) both lactose and glucose are present d.) lactose is present, glucose is absent, but there is a mutation to the sequence in the CAP site

Describe the effect of a mutation in the gene that encodes for the C3 protein of...

Describe the effect of a mutation in the gene that encodes for the C3 protein of the complement pathway.

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