a. what is the difference between a flourescence
excitation spectrum and a fluorescence emission spectrum?. which
resembles an absorption spectrum.
b. consider a molecule that can fluoresce from the s1
state and phosphoresce from the t1state. which is emitted at longer
wavelength?make a sketch showing absorption fluorescence and
phosphorescence on a single spectrum.
Compare and discuss the relative advantages and disadvantages to
using circular dichroism or fluorescence spectroscopy or light
scattering to study
1. protein folding
2. protein-ligand interactions
3. protein structure
Explain why fixed tissues can be used to perform fluorescence
in situ hybridisation but not karyotyping. Compare what is
being detected in each method and how samples are prepared.
(a) Explain the features of the spectra of alpha, beta and gamma
radiation.
(b) Why does alpha/beta spectroscopy have to be conducted in an
environment different from that for gamma spectroscopy?
Which spectroscopy is more sensitive: fluorescence or
absorption? Why? (2) Between fluorescence and phosphorescence:
which one has a higher intensity? Which one lasts longer?