Question

Explain how flow cytometry would be beneficial for physicians on the pursuit for personalized medicine.

Answer 1

Flow Cytometry is the process in which cells in a isotonic buffer suspension passes through a laser beam one by one.Flow Cytometry provides a well established method to identify cells in solution and used for evoluating peripheral blood, bone marrow and other body fluids.(that is, It is most Comment used for Immunophenotyping-The technique which identifies and quantifies populations of cells in a heterogeneous sample usually blood, bone marrow).

# It would be beneficial for physicans on the pursuit for personalized medicine by considering its use-

*It is a multi parametric data-gatheringwith an enormous, well established array of applications for organelles, lysed cell contents, intact cells etc.. That is, it has the potential to yield a distribution of cellular phenotypes with high dimensionality specific to a disease state.

*Purified populations of the cells which represents disease state can be collected to characterise biomolecules.

*Cytometry can be outfitted with multiwell plate accessories to provide single cell capture of specific cells with correlation to cells individual phenotype (or) even rapid sampling from microtiter plates.

*New innovations in cytometry allows determining biomarkers from individual cells. Can use mass Spectroscopy to record elements composition and biomarkers distribution of cells at a rate of about 1000 cells per second.

*It is used in screening of Blood cancer.