Question

Based on your previous experience on the AA, what tends to happen to the calibration curve when more concentrated standards are used? Do you expect this to happen for this experiment with AE and (ICP-OES) ? Why or why not?
in AA when more concentrated standards are used, the absorbance increased.

Atomic Absorption (AA),
Atomic Emission (AE),
and Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES)

Answer 1

With high concentration samples, the problem of pre-atomization analyte loss occurs during the char step. This phenomenon causes an analytical result that is biased low. This loss can occur even when using a matrix modifier and a conservative char temperature. Preatomization loss is indicated by an absorbance less than zero at the beginning of the read cycle and ending with the peak’s tail dipping below the baseline.

Analytes at high levels in the analytical solution may not be volatilized out of the graphite furnace. This residual analyte may have a “memory effect” on the next measurement resulting in a falsely high result (i.e., false positive or high bias).

While ICP and AES are not affected by high concentration samples because of the reason that their characterisation is based on the atoms the samples provide not on the concentration of the samples.