Question

In: Biology

Working with neurons in the lab, you noticed that a batch of isolated neurons from Science...

Working with neurons in the lab, you noticed that a batch of isolated neurons from Science Express is not working appropriately. Your research advisor asks you to sort it out. Doing several experiments you noticed that most neurons either failed to start or failed to get back to the resting phase (state). You suspect that the special batch of neurons might carry a mutation and was mistakenly sent by this company. What kind of mutation do you suspect these neurons carry? Include in your discussion how to process would work for wild type neurons.

Solutions

Expert Solution

If your cells fail to enter G0 phase, this means they keep dividing uncontrollably. However, by the way the question is posed, it would seem tht they are initially in G0, then you induce division (common for cell culture, I assume), and then you are not able to halt it. This has Tumor Suppressor Genes written all over it.

The Tumor suppresors (TSG, for short) have the task of halting and resuming cell cyle at certain stages. P53 and Rb proteins are the flagships of TSGs, being in charge of stopping division to check for errors in the new DNA strands or to cause quiescence in mature and stem cells. Your cells probably contain a mutated P53 or Rb gene (or both), which results in them not being able to stop dividing and thus, will never enter the G0 phase. To test this hypothesis you can extrat DNA and sequence using primers for the P53/Rb gene and see if they are mutated.

I am not quite sure of what you are trying to ask in the second qustion, but I will assume it is about how the process of entering G0 happens in regular (Wild Type) neurons, and it is quite simple:

Neurons are a type of cell that does not divide, safe for a few neurogenic sports in the brain. This means that, once they are mature, fully differentiated cells, they will enter G0 and stay that way until they die. In a culture, neurons should divide until they are fully differentiated, which I assume is what yu expect for these experiments, and then stop dividing.


Related Solutions

Anissa is working in an electrophysiology lab. She has isolated an excitable patch of membrane in...
Anissa is working in an electrophysiology lab. She has isolated an excitable patch of membrane in the cell, generates one action potential but then finds that she cannot generate any action potentials, even with the supre-threshold stimulation. Which of the following statements could most likely account for this scenario? a) The cell Anissa is recording does not contain voltage gated Na+ channels b) The intracellular concentration of K+ ions is now higher than the extracellular concentration c) The membrane contains...
A student is a lab isolated DNA from a transgenic mouse, a model used to study...
A student is a lab isolated DNA from a transgenic mouse, a model used to study heart failure and wanted to perform the following: 1- Amplify DNA 2- Clone the gene of interest 3- Sequence the sequence of the gene that causes heart failure What does he need to do for each step
You are a microbiologist working on a recently isolated species of bacteria. You start a culture...
You are a microbiologist working on a recently isolated species of bacteria. You start a culture of the bacterium growing at 15 degrees Celsius. In oder to study the membrane composition of this species, you then increase the growth temperature to 37 degrees Celsius. If this bacterium were "typical" what would you expect to see with regard to the fatty acid conpostion of the membrane? Why?
(1) Pre Lab Question: You are working in the lab and are told to create an...
(1) Pre Lab Question: You are working in the lab and are told to create an artificial membrane from phospholipids. You are then instructed to add certain solutes to the membrane to see if it works appropriately. Rank the permeability for the following across a phospholipid membrane for the following: lysine, urea, sodium ion, insulin, water, indole
You have isolated a strain of brewer’s yeast (Saccharomyces cerevisiae) in the lab for your second...
You have isolated a strain of brewer’s yeast (Saccharomyces cerevisiae) in the lab for your second job at a hip new microbrew . You find that this strain ferments more efficiently and adds a superior flavor profile to your brews. You want to clone the strain of yeast and generate a genomic library to determine the genes responsible for this finding. To do so you: Obtain fragments of the whole yeast genome (DNA) through restriction digestion Open your vector and...
You begin working in a research lab on campus. In the lab's freezer you find a...
You begin working in a research lab on campus. In the lab's freezer you find a tube of preserved bacterial cells. You want to grow the cells, but you do not know what their nutritional requirements are. What type of growth medium would be best? Could someone please explain to me why this is the correct answer? I am still unsure on whether I fully understand complex and define (minimal) media as well. Incorrect Minimal medium supplemented with only essential...
Nature of Science and lab safety What makes one statement or claim science? Along these lines,...
Nature of Science and lab safety What makes one statement or claim science? Along these lines, how do you distinguish a scientific claim from a non-scientific claim, or pseudoscience?
Even though strychnine can easily be isolated from the Nux Vomicus tree, chemists have been working...
Even though strychnine can easily be isolated from the Nux Vomicus tree, chemists have been working on the problem of synthesizng strychnine in the laboratory since the 1950s. As of 2012, the molecule had been synthesized using 17 different strategies. Please comment on the motivation for all of this research- why did chemists originally want to synthesize strychnine? Why did chemists continue to pursue strychnine synthesis research years after it had been done the first time? Should chemists still be...
You have recently joined a lab and will start working with J558 cells today. You are...
You have recently joined a lab and will start working with J558 cells today. You are handed a T25 flask (5 mL) containing these cells, so you take an aliquot of the cells (using aseptic technique) and dilute the aliquot with Trypan Blue in a 1:1 ratio. After loading the hemocytometer and observing the cells under the microscope, you count the cells and obtain the following values: Quadrant: Transparent cells: Blue cells: Q1 52 1 Q2 60 3 Q3 58...
You are an experienced, certified nurse working in an endoscopy lab. You have been asked to...
You are an experienced, certified nurse working in an endoscopy lab. You have been asked to prepare a presentation for staff education days at your facility. Select a digestive disorder for this discussion and, if necessary, research the clinical manifestations of the disorder. Describe the alterations in digestive functioning associated with the disorder you have chosen. Be sure to present it in a format that is appropriate for the type of presentation you are giving and to the audience you...
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT