Question

You have isolated C57BL6 trophoblast stem cells and have propagated them on MEFs with heparin. To test for the success of your culture protocol, you are injecting the cells into a C57BL6 blastocyst. Once the pups are born, you notice that your experiment failed. Why? What was the expected outcome of this injection?

the answers given for this question by previous individuals do not give the correct answer. can someone please explain this clearly and concise

Answer 1

TS-like cells could also be derived in CDM containing FAY , but these cells exhibited a rather differentiated cellular morphology and a slower proliferation rate. These cells initially coexisted with TS cells, but because they proliferated more rapidly, they ultimately predominated over the TS cells. TS cells self-renewal capacity, marker gene expression, differentiation competence into various trophoblast lineage cells in vitro, and the ability to contribute to the placenta in chimeras. The isolation of stable trophoblast stem (TS) cell lines from early mouse embryos has provided a useful cell culture model to study trophoblast development. TS cells are derived from pre-implantation blastocysts or from the extraembryonic ectoderm of early post-implantation embryos. The derivation and maintenance of mouse TS cells is dependent upon continuous fibroblast growth factor (FGF) signaling. Gene expression analysis, differentiation in culture, and chimera formation show that TS cells accurately model the mouse trophoblast lineage.