Part A - Short Answer If tryptophan (trp) codons containing two trp codons in its leader...

Part A - Short Answer

If tryptophan (trp) codons containing two trp codons in its leader region were mutated to serine (ser) codons, then a high level of expression of the mutated operon would be visible ONLY if trp and ser were absent at the same time from the media. Explain why this occurs.

Part B - Multiple Choice

Circle the correct relationship between the trp operon and the repressor.

The repressor acts in 'cis'.
The repressor protein is responsible for the formation of the "termination" stem loop.
Binding of the repressor protein leads to attenuation of the trp operon.
The presence of tryptophan results in increased expression of the tryptophan gene
When bound to tryptophan, the repressor is able to bind the operator of the trp operon

Part C - Multiple Choice

Before transcription of the lac operon can commence, RNA polymerase must strongly bind to the promoter. Circle the correct statement as to why this occurs.

lacI+ is mutated to lacI-
a CAP-cAMP complex binds to the CAP site in the promoter in the absence of glucose.
CAP binds to the CAP site in the promoter in the presence of glucose.
catabolite repression occurs in the presence of glucose.
catabolite repression occurs in the absence of glucose.

Expert Solution

Please find the attached answers of given parts

gladiator answered 1 year ago

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