Radiolabeled NADH was added to cell extracts containing actively respiring mitochondria. After a short incubation period,...

Radiolabeled NADH was added to cell extracts containing actively respiring mitochondria. After a short

incubation period, mitochondria were isolated by subcellular fractionation using density gradient

centrifugation and analyzed for radioactivity. In one experiment, tritium labelled NADH (3H-NADH) was used.

14C-NADH was used in a second experiment. Radioactivity was associated with mitochondria in only one of

these experiments. Which one? Explain.

Expert Solution

gladiator answered 2 years ago

When the antibiotic valinomycin is added to actively respiring mitochondria, several things happen; the yield of...

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