Question

To do electrophoresis, you will need to make a solution called TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. Assuming no errors and no spills, you will need a minimum of 50 ml 1X TAE to make your agarose gel plus 1 liter of 1X TAE for gel running buffer. TAE is normally made as a 50X concentrated stock.

8. What is the minimum volume of 50X TAE that you will need to make?

Answer 1

Ans:- Here Concentration of TAE C₁ = 50X

For agarose solution

Concentration C₂ =1X

Volume of agarose solution V₂ = 50ml

Now, minimum volume for TAE V₁ = C₂V₂ /V₁

                                                  = 1x50/50

                                                   = 1ml

For gel running buffer

Volume V₂ = 1L = 1000ml

Concentration C₂ = 1X

minimum volume V₁ = C₂V₂/C₁

                               = 1x1000/50

                                  = 20ml

Therefore the minimum volume for agarose gel is 1ml and for gel running buffer is 20ml