does methotrexate inhibit folic acid synthesis or Thymidylate synthase?

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According to the given question-

Methotrexate is work as antagonist of folate .Methotrexate responsible for inhibiting the enzyme DHFR or dihydrofolate reductase catalyzes the reduction of folic acid into tetrahydrofolic acid.
This Tetrahydrofolate is regenerated by dihydrofolate reductase required for purine as well as thymidylate nucleotide biosynthesis.
methotrexate inhibit the activity of dihydrofolate reductase and this causes thymidylate and purines deficiency results in decrease rate of synthesis of nucleic acid . so we can say that methotrexate play an important role in several process such as cellular replication, DNA synthesis, and DNA repair.
So methotrexate causes -
- dihydrofolate reductase inhibition
- thymidylate synthase inhibition
- and reduced folate transportation.
methotrexate have high affinity for binding with dihydrofolate reductase compared to folic acid .
Thymidylate synthetase enzyme convert uridine 5′-phosphate into thymidine 5′-phosphate. Cofactor methylenete trahydrofolate work as donor for formaldehyde and oxidized to dihydrofolate as well as reduced to tetrahydrofolate by dihydrofolate reductase enzyme.

gladiator answered 2 years ago

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