Question
In: Biology
Draw peptide PLACE at pH 3 and describe how you would separate it using cation/anion exchange...
Draw peptide PLACE at pH 3 and describe how you would separate it using cation/anion exchange column with the pH of the buffer and order of elution.
Solutions
gladiator answered 2 years agoRelated Solutions
An anion-exchange resin can be used instead of the cation-exchange resin in this experiment to separate...
An anion-exchange resin can be used instead of the
cation-exchange resin in this experiment to separate the three
amino acids in the known mixture. What changes to experimental
protocol are required to achieve complete resolution? Support your
response with an explanation. (HINT: consider pH).
You have been provided a mixture containing four amino acids
(Glu, His, Pro and Lys) to be separated by ion-exchange
chromatography using a Dowex 50 cation-exchange, prepared in a
loading buffer at pH 2.0. The following...
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3. (a) What is SDS (sodium dodecylsulfate)? Draw the
structure
(b) How would you separate a mixture of polypeptides according
to their lengths using SDS –
polyacrylamide gel electrophoresis? Describe in a few
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You have a mixture of proteins with the following properties, using a cation exchange column, what...
You have a mixture of proteins with the following properties,
using a cation exchange column, what would be the order of elution
for the following mixture of proteins
MW pHI
A. 85,000 7.0
B. 65,000 6.2
C. 35,000 9.5
Describe how you could use pH control to separate lead and iron if they were both...
Describe how you could use pH
control to separate lead and iron if they were both precipitated as
their hydroxides. (Hint: how could pH be used to dissolve one of
the hydroxide precipitates but not the other?)
How to place the same image in 4 separate divs by pressing a button using a...
How to place the same image in 4 separate divs by pressing a
button using a javascript function?
HTML AND CSS:
<html>
<head>
<meta charset="utf-8">
<style>
body {
margin: 0;
}
#flex-container {
display: flex;
justify-content: space-between;
height: 300px;
}
nav {
background-color: royalblue;
height: 75px;
text-align:center;
}
#button {
}
header {
background-color: lightskyblue;
height: 400px;
}
section {
background-color: gray;
margin: 75px 100px;
/*height:650px;*/
padding-bottom:50px;
}
footer {
background-color: black;
height: 100px;
}
p {
background-color:...
How can one extract a negatively charged protein from a cation exchange column other than using...
How can one extract a negatively charged protein from
a cation exchange column other than using NaCl?
Explain how you would separate a solid mixture of sand and salt using any of the...
Explain how you would separate a solid mixture of sand and salt
using any of the following methods either distillation,
chromatography, or crystallization.
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How would you describe your own ideological beliefs? Where would
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private financial reporting? Explain.
writing pretend you are working in a hotel busness place describe how would you apply use...
writing pretend you are working in a hotel busness place
describe how would you apply use ethic to be successfull as an
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