EtOH+ NAD+ ---> Acetylaldehyde + NADH initial concentrations: EtOH= 900uL (45.0mM ethanol in 50.0mM Tris Buffer)...

EtOH+ NAD+ ---> Acetylaldehyde + NADH

initial concentrations: EtOH= 900uL (45.0mM ethanol in 50.0mM Tris Buffer)

NAD+= 900uL (0.290mM NAD+ in 50mM Tris)

ADH= 100uL (0.0503 g ADH in 7,000. uL) (doesn't apply to Keq equation)

The average absorbance at equilibrium for this mixture is 0.3602 at 342.7nm

My experimental molar extinction coefficient= 5281.2821 M-1 cm-1, path length=1cm

What is the equilibrium constant? Complete the ICE table and calculate the Keq of the reaction from the information provided.

Expert Solution

queen_honey_blossom answered 2 years ago

947.Ethanol metabolism in the liver results in the increase of the NADH/NAD+ ratio in the liver...

947.Ethanol metabolism in the liver results in the increase of the NADH/NAD+ ratio in the liver cytosol. 946.In gluconeogenesis, the regulatory enzyme fructose 1,6-bisphosphatase is inhibited by AMP. 945.Glucose-6-P dehydrogenase deficiency can lead to drug-induced hemolytic anemia because not enough NADH is generated. 944.Glucagon and epinephrine are similar in that both promote glycogenolysis. 943.Phosphofructokinase activity can be decreased by AMP. 942.Protein phosphatase can reverse the action of the kinase on enzymes.

In a NAD+ ------> NADH reduction, the molecule NAD+ is the ______________ when it gets reduced to NADH.

In a NAD+ ------> NADH reduction, the molecule NAD+ is the ______________ when it gets reduced to NADH. The ___________ that attacks is __________.

You need to prepare a buffer with the following concentrations: 0.2 M Tris (FW Tris Base...

You need to prepare a buffer with the following concentrations: 0.2 M Tris (FW Tris Base = 121.1), 0.2 M NaCl (FW NaCl = 58.44), 0.01 M MgAc (FW MgAc = 214.4) and 5% glycerol. Prepare a table that shows how to prepare 1 L of this solution.

Describe NAD+, NADH, NADP+, NADPH. And how NADH is used for catabolism mainly, while NADPH is...

Describe NAD+, NADH, NADP+, NADPH. And how NADH is used for catabolism mainly, while NADPH is used for biosynthesis.

Describe how to prepare for this desired Tris buffer: 500 ml of 2 M Tris-HCl buffer...

Describe how to prepare for this desired Tris buffer: 500 ml of 2 M Tris-HCl buffer with a pH of 9.2 . The molecular weight of Tris base is 121.14 g/mol.

Solutions are made with the folowing initial sets of concentrations. Which will not form a buffer...

Solutions are made with the folowing initial sets of concentrations. Which will not form a buffer solution 0.1 M HCN and 0.2 M KCN 0.2 M HCN and 0.1 M NaOH 0.1 M HCN and 0.2 M NaOH 0.2 M HCN and 0.1 M KCN

Ethanol (EtOH) can be produced by the hydration of ethylene, C2H4 + H2O ? C2H5OH, at...

Ethanol (EtOH) can be produced by the hydration of ethylene, C2H4 + H2O ? C2H5OH, at 300?C and high pressure with a phosphoric acid catalyst. Downstream of the reactor, a condenser is used to separate the product, which contains only EtOH and H2O, from a recycle stream that mixes with the fresh feed before entering the reactor. The fresh feed contains 30 mol% C2H4 and the remainder is steam. The single pass conversion is 20%. a. Draw and label the...

You add a drug that oxidizes NADH to NAD+ IMMEDIATELY after it is produced in the...

You add a drug that oxidizes NADH to NAD+ IMMEDIATELY after it is produced in the Krebs Cycle. What would happen to the production of ATP by chemiosmosis. Explain your reasoning

Glycolysis and fatty acid oxidation are both affected by the [NADH]/[NAD+] ratio in the cell. If...

Glycolysis and fatty acid oxidation are both affected by the [NADH]/[NAD+] ratio in the cell. If the conditions inside a cell are such that [NADH] >>[NAD+] what will be the effect on both pathways? Be sure to explain the direct connection between [NADH] and specific reactions in these and related pathways.

1. How would a sudden increase in the [NADH]/[NAD+] ratio effect the operation of the Krebs...

1. How would a sudden increase in the [NADH]/[NAD+] ratio effect the operation of the Krebs Cycle? Explain. 2. Why is the citric acid cycle considered amphibolic? Explain by giving examples.

Question

EtOH+ NAD+ ---> Acetylaldehyde + NADH initial concentrations: EtOH= 900uL (45.0mM ethanol in 50.0mM Tris Buffer)...

Solutions

Expert Solution

Related Solutions