What are the three basic steps in the programming of Polymerase Chain Reaction (PCR) process?

Expert Solution

The three basic steps in Polymerase Chain Reaction (PCR) are:

1. Denaturation: In a double stranded template DNA, the two strands are required to be seperated like during replication process. Denaturation is carried out by raising the temperature to 94-96°C at which complementary hydrogen bonds break off and denaturation occurs.

2. Annealing: At this step, the primers bind to the target single stranded DNA sequences. The temperatures are lowered at this step to critical temperature (55-65°C). The annealing conditions are usually optimized beforehand.

3. Extension: Using the template strands DNA Polymerase enzyme extends the oligonucleotides by adding dNTPS as dictated by the template. The optimal temperature for extension by DNA synthesis using (Taq) DNA polymerase is around 72°C.

The three steps are repeated in cycle.

The three basic steps in Polymerase Chain Reaction (PCR) are: Denaturation, Annealing and Extension

Shams answered 2 years ago

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