This is a three part question: a) What are some major differences between making transgenic mice...

This is a three part question:

a) What are some major differences between making transgenic mice by microinjection and creating knockout mice by homologous recombination (before the advent of CRISPR/Cas9 technology)?

b) Explain how negative and positive selection work when creating a knockout mouse. Why are both types of selection useful?

c) Why is the creation of knockout mice so much easier when using CRISPR/Cas 9 genome editing?

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gladiator answered 1 year ago

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