In: Biology
This is a three part question:
a) What are some major differences between making transgenic mice by microinjection and creating knockout mice by homologous recombination (before the advent of CRISPR/Cas9 technology)?
b) Explain how negative and positive selection work when creating a knockout mouse. Why are both types of selection useful?
c) Why is the creation of knockout mice so much easier when using CRISPR/Cas 9 genome editing?