The most important methods to determine the size of nanoparticles are TEM and AFM. What are...

The most important methods to determine the size of nanoparticles are TEM and AFM. What are the advantages and disadvantages of the two methods?

Expert Solution

Hey there!

The physical basis of measuring the size of the particles in (Transmission Electron Microscope)TEM is by "scattering of electrons" through the sample. TEM works in an environment which is a vacuum. TEM goes with the principle , that when a beam of electrons interact with the specimen. The material sensitivity in TEM increases with increasing atomic number of the specimen. Hence, results with higher scattering in the formation of the image. One disadvantage of TEM is that smaller nanoparticles without coating for the observation makes it impossible to get into the image.TEM offers the largest throughput and it is also useful for rapid characterization. Another drawback is that TEM requires coating of the sample and hence will result with high cost to conduct experiments and observation. It may even go upto 20 times the cost of what it takes than AFM.

When it comes to (Atomic Force Microscopy) AFM the physical basis is by "physical interaction with sample". AFM works in any environment, that is in air or liquid or vacuum. AFM is considerably different when compared other microscopic techniques, because, AFM collects all 3 spacial dimension information when compared to TEM. AFM takes information in both x-y plane and also the depth with z-axis. AFM images have high resolutions with lesser noise level. When it comes to drawbacks, AFM doesn't have a fast scanning time. The scanning takes place with help of a probe. This probe sends in laser beams which reflects off from a cantilever. This laser beam interacts with the substrate . Because of this setting, up and down operation takes place which results with difficulty in measuring the overhangs and steepwalls through AFM.

I hope the explanation helps.. Cheers :)

genius_generous answered 2 years ago

Silica nanoparticles are synthesized through the sol-gel method. The size of the nanoparticles is controlled by...

Silica nanoparticles are synthesized through the sol-gel method. The size of the nanoparticles is controlled by the amount of water. Why is the size of nanoparticles controlled by the amount of water?

Identify the synthesis method that has the best chance to yield nanoparticles of a uniform size....

Identify the synthesis method that has the best chance to yield nanoparticles of a uniform size. For comparison, also identify which method we covered that you believe is the least likely to yield uniformly sized particles. Explain your reasoning for selecting these two.

what is the most important thing of opportunity cost? what is the most important thing of...

what is the most important thing of opportunity cost? what is the most important thing of scarcity in economic ?

a) Why is it important to evaluate the effects of nanoparticles on bacteria? b) How do...

a) Why is it important to evaluate the effects of nanoparticles on bacteria? b) How do nanoparticles cause nucleic acid damage?

What are those four most important factors determine supply of loanable funds? What are those four...

What are those four most important factors determine supply of loanable funds? What are those four important factors determine the changes in the supply of loanable funds?

from QFD, what factor use to determine most important design specification? Is it the absolute weight...

from QFD, what factor use to determine most important design specification? Is it the absolute weight or relative weight? How can I list the order of importance of the design specification using data from QFD?

Discuss the two most common methods used to determine when the economy is in a recession.

1. Protein crystallography is one of the most common methods used to determine the structure of...

1. Protein crystallography is one of the most common methods used to determine the structure of proteins. However, an emerging method called “Cryo-EM” is used to determine the structure of the spike protein. In your own words, describe what “Cryo-EM” is, and why it’s a useful for studying protein structures. 2. The spike protein is heavily glycosylated. Briefly describe what glycosylation is, and why it might be useful for the virus. 3. Is the spike protein structure a Primary, Secondary,...

One of the most popular methods for determine trace amounts of water is a coulometric titration,...

One of the most popular methods for determine trace amounts of water is a coulometric titration, called the Karl Fischer. Measuring trace amounts of water is one of the most challenging analytical methods. a) What would be another method that would measure larger amounts of water contained in a sample, and how would you do it? b) The Karl Fischer method is a secondary coulometric analysis that utilizies the generation of I2/I3 with starch, just like you did in lab,...

What are most important lipids, carbohydrate, or protein? Which is most important for our health and...

What are most important lipids, carbohydrate, or protein? Which is most important for our health and why? Can we live a productive life if we drop lipids, carbohydrates, or protein from lives? Please be as detailed as possible. DON'T use older answers on chegg, because they are not accurate. If you going to write it, please be neat. Thank you!

Question