1. The chicken ovalbumin gene has five introns and interrupting the coding sequences or exons. How...

1. The chicken ovalbumin gene has five introns and interrupting the coding sequences or exons. How many R-loops will be observed if DNA of the gene and cytoplasmic mRNA are mixed under conditions that allow hybridization? How many R-loops will be observed if nuclear pre-mRNA is used?

Expert Solution

R-loops or recombination loops are the physical loops formed during mRNA processing in order to perform two main functions:

Removal of introns from the mRNA
Recombination of the exons to give rise to novel codon sequences, hence proteins

The number of R-loops formed in a nascent mRNA depends upon the number of introns present in it. Since all the introns are required to be absolutely removed, all the introns contribute to formation of a R-loop. Hence, number of R-loops remains equal to the number of introns. Further, each intron resides between two exons, hence, the number of introns must be technically one less than the number of exons.

Thus, according to the information, the number of introns in chicken ovalbumin gene is 5, thus total 5 R-loops will be formed in the cytoplasmic mRNA. Importantly, the pre-mRNA or nuclear mRNA does not undergo splicing, hence no R-loops will be formed in it.

gladiator answered 1 year ago

Gene is: LOC_Os05g38000 The gene name and chromosomal location. (Oryzabase) Number of exons and introns. (RGAP)...

Gene is: LOC_Os05g38000 The gene name and chromosomal location. (Oryzabase) Number of exons and introns. (RGAP) Describe the expression pattern. This will require some research as there is no convienent Atlas. Can someone please help with this question!

1) You obtain the sequence of a gene containing 10 exons, 9 introns, and a 3′...

1) You obtain the sequence of a gene containing 10 exons, 9 introns, and a 3′ UTR containing a polyadenylation consensus sequence. The fifth intron also contains a polyadenylation site. To test whether both polyadenylation sites are used, you isolate mRNA and find a longer transcript from muscle tissue and a shorter transcript from all other tissues. Briefly speculate about the mechanism involved in the production of these different transcripts. 2) Briefly speculate about why plants deficient in Dicer activity...

how do non protein coding sequences affect gene expression and protein activity

2. Draw a eukaryotic gene with 5 exons, indicating all sequences needed for transcription, RNA processing...

2. Draw a eukaryotic gene with 5 exons, indicating all sequences needed for transcription, RNA processing and translation. Draw the same gene as an mRNA, post processing, indicating all regions that function for stability and translation.

You are studying a gene that has four exons. You find that in some cell types...

You are studying a gene that has four exons. You find that in some cell types the mature mRNA contains all four exons while in others exon 3 is missing from the transcript. You suspect that cells that lack exon 3 express a splicing repressor. Which splice site do you think this repressor acts on to inhibit inclusion of exon 3 (include a drawing)? Explain your reasoning.

Describe how a single nucleotide insertion in the coding sequence of a gene would affect the...

Describe how a single nucleotide insertion in the coding sequence of a gene would affect the production of the protein (polypeptide) the gene encodes.

A Drosophila transgene has coding sequences for GFP positioned downstream of a 7-kb DNA fragment taken...

A Drosophila transgene has coding sequences for GFP positioned downstream of a 7-kb DNA fragment taken from the segment-polarity gene wingless (wg). In Drosophila embryos, the transgene expresses GFP fluorescence in exactly the same pattern as Wg. Crosses are used to place the transgene in different mutant backgrounds. Describe the pattern of GFP fluorescence you expect to see in each of the following types of embryos if they are examined at germ-band extension. i. wild-type ii. embryos laid by a...

1) Describe the use of Small SubUnit ribosomal RNA (SSU rRNA) gene sequences for the identification...

1) Describe the use of Small SubUnit ribosomal RNA (SSU rRNA) gene sequences for the identification of bacteria in microbial communities. 100 marks 2) Describe how the genetic content of a bacterium determines the bacterium’s activities and behaviours. 100 marks

Question 1: In the NCBI database, retrieve the nucleotide sequences NG011676. Report: Gene name, database name....

Question 1: In the NCBI database, retrieve the nucleotide sequences NG011676. Report: Gene name, database name. Number of exons and its position (the start and the end of each exon). The start and the end of coding region (CDS) Accession number of protein from this gene and the length of polypeptide. Question 2: Use NG011676 to run GenScan. Report the results and compare the results with information of this gene from Question 1. Question 3: Use NG011676 to run FGENESH...

1.Explain to a layperson how medical coding works. What is the purpose of medical coding? Include...

1.Explain to a layperson how medical coding works. What is the purpose of medical coding? Include CPT codes, HCPCS, and ICD-10 codes. 2. explain medical coding and billing to a person not in the healthcare field

Question