If you had ddGTP used in sanger sequencing, describe how the nucleotide would be different than dGTP. Why is it important in Sanger sequencing? Be detailed in your repsonse.

Can anyone help with this question?

A solution containing 0.10 M Zn²⁺ and 0.10 M Fe²⁺ is saturated with H₂S (i.e. 0.10 M H₂S). (a) What must the H+ concentration be to separate these ions by selectively precipitating ZnS? Note: ZnS has Ksp=1.2x10^-23; FeS has Ksp=3.7x10^-19. (b) What is the smallest concentration of Zn²⁺ that can be achieved without precipitating any of the Fe²⁺ as FeS?

A solution consists of 44.3 g of hydrochloric acid (HCl), 75.7 g water, and has a volume of 101 mL.

Calculate the weight percent of HCl, the molality of HCl, the mole fraction of HCl, and the molarity of HCl in the solution.

A solution of barium chloride (BaCl₂) in water is 5.000 % barium chloride by mass. Its density is 1.0434 g cm^-3 at 20 °C. Compute its molarity, mole fraction, and molality at this temperature.

Molarity BaCl₂ =  

Mole fraction BaCl₂ =

Molality BaCl₂ =  

The EPA's secondary standards for contaminants that may cause cosmetic or aesthetic effects in drinking water suggest an upper limit of 5.00×10^-2 mg/L for manganese . If 1.40×10⁴ liters of water in a storage tank contains 2.66 grams of Mn, what is the contaminant level in ppm? Is this level acceptable based on EPA guidelines?

ppm   _________acceptable/not acceptable

Colligative properties (boiling point increase)
a. Order the following solutions in ascending order of their boiling point: 0.0825 m NaCl, 0.050 m KCl, 0.050 M Na2SO4, 0.055m Ca (NO3) 2, 0.050 m Na3PO4.

If the boiling point of a 0.100 m NaCl aqueous solution is 100.5 ◦C, answer…
b. How much will the boiling point of a 0.0825 m KCl solution be?
c. How much will be the boiling point of a solution 0.0825 m Ca (NO3) 2?
d. Determine the osmotic pressure of the solutions in part a.

Questions and Problems

1. Complete the table containing the summary of data below.

Summary of Solubility and Ksp Data

Sat’d Ca(IO3)2 in: Solubility (M) _______ Ksp (M3 ) ___________T˚C ___________

H2O: Solubility (M) _______ Ksp (M3 ) ___________T˚C ___________

0.0100 M KIO3 : Solubility (M) _______ Ksp (M3 ) ___________T˚C ___________

0.0100 M KCl:  Solubility (M) _______ Ksp (M3 ) ___________T˚C ___________

2. Compare the values of Ksp of Ca(IO3)2 obtained for the three solvents.

(a) Which two are most nearly the same? Briefly explain. (

b) What value of Ksp would you expect for Ca(IO3)2 dissolved in 0.0100 M NaNO3 at the same temperature?

3. Is the solubility of Ca(IO3)2 greater in pure water or in 0.0100 M KCl? Briefly explain. {Hint: Consider the relative magnitudes of the ion-dipole and ion-ion forces experienced by the Ca 2+ and IO3 - ions in the two solutions.}

Chemical Equilibria: Ksp of Calcium Iodate Report Form -10

4. Calculate the molar solubility, s, of calcium iodate in 0.020 M Ca(NO3)2, a completely dissociated strong electrolyte. {NO3 - ion does not chemically interact with either Ca2+ or IO3 - .} Assume that Ksp for Ca(IO3)2 = 2.0 x 10-6 .

To set up the problem, we can write the following equations:

Material balance for calcium: [Ca2+] = s + 0.020

Material balance for iodate: [IO3 - ] = 2s

Ksp = 2.0 x 10-6 = [Ca2+][IO3 - ] 2 = (s + 0.020)(2s)2

Here in the last equation we have the solubility in s, but s is not negligible when compared to 0.020 M.

So here we are confronted with a cubic equation in s! To simplify the solution to this equation we can rearrange it into a more useful form and obtain s by successive approximations (iteration).

Solving the equation for s2 we get, s^2 = Ksp/ 4(s + 0.020) or s = 1 /2 [Ksp/( s + 0.020 )^]1/ 2

We have to find the value of s that makes both sides of the equation equal, that is the value of s we insert on the right side will yield the same value on the left side. We can start by neglecting s on the right side calculating s on the left side. The value of s obtained is then inserted on the right side and a new value of s is calculated on the left side. This iteration process is continued until two consecutive values of s calculated are the same within the precision of the measurement (significant figures).

Calculate the pH at the following intervals if 30mL 0.20M of formic acid, HCHO₂ is titrated with 0.15M NaOH. For HCHO₂ K_a = 1.8 x 10^-4

a) before any NaOH has been added

b) after a total of 15.0mL of NaOH has been added

c) at the equivalence point

d) after a total of 50.0mL of NaOH has been added.

When 22.0 mL of a 5.89×10-4 M sodium hydroxide solution is combined with 12.0 mL of a 5.49×10-4 M silver fluoride solution does a precipitate form? (yes or no) For these conditions the Reaction Quotient, Q, is equal to

An X-ray photon with a wavelength of 0.979 nm strikes a surface. The emitted electron has a kinetic energy of 984 eV.

What is the binding energy of the electron in kJ/mol? [Note that KE = 12mv2 and 1 electron volt (eV) = 1.602×10−19J.]

An adiabatic tank contains 120 kg of liquid water initially at 70 °C. The water within the tank is utilized to quench (cool) a 15 kg steel block initially at 300 °C. The steel block is dropped into the tank, and after some time the steel block and the water in the tank reach thermal equilibrium.

Determine the total entropy change during the quenching process.

Specific heat of steel : C_steel= 0.50 kJ/kg*K.

Specific heat of water : C_water= 4.18 kJ/kg*K

A 0.4713 g sample of pewter, containing tin, lead, copper, and zinc, was dissolved in acid. Tin was precipitated as SnO2·4H2O and removed by filtration. The resulting filtrate and washings were diluted to a total volume of 250.0 mL. A 15.00 mL aliquot of this solution was buffered, and titration of the lead, copper, and zinc in solution required 35.63 mL of 0.001504 M EDTA. Thiosulfate was used to mask the copper in a second 20.00 mL aliquot. Titration of the lead and zinc in this aliquot required 34.75 mL of the EDTA solution. Finally, cyanide was used to mask the copper and the zinc in a third 25.00 mL aliquot. Titration of the lead in this aliquot required 26.61 mL of the EDTA solution. Determine the percent composition by mass of each metal in the pewter sample.

The initial rates listed in the following table were measured for the reaction between nitrogen dioxide and carbon monoxide that occurs in engine exhaust.

NO2(g)+CO(g)→NO(g)+CO2(g)NO2(g)+CO(g)→NO(g)+CO2(g)

What is the value of the rate constant and its units?

Express your answer to three significant figures and include the appropriate units.

Which of these has the lower concentration of hydrogen ions: 0.1 M H2SO4, 0.1 M HCl, CaCO3, or pure water?

How many liters of water have fallen on 1 hectare of land if 2.5 inches of water fell
during a rain storm? Give your answer in the correct number of significant digits