An enzyme is discovered that catalyzes the chemical reaction

SAD --> <-- HAPPY

A team of motivated researchers set out to study the enzyme, which they call happyase. Under a constant enzyme concentration, [E] of 2.0 uM, they studied the relationship between substrate concentration [SAD] and reaction velocity V₀. Their data is shown below:

[SAD] (uM)                V₀ (uMs^-1)      

20                    3.4 x 10³

40                   5.4 x 10³

60                   6.6 x 10³

80                    7.4 x 10³

Can you determine K_cat and K_m from the above data?

2. While [E] remains 2.0 um and [SAD] = 12 uM, what would the reaction velocity V₀ be?

3. In a separate happyase experiment using [E] = 1.0 uM, the reaction velocity, V_0, is measured as 3.6 x 10³ uMs^-1. What is the [SAD] used in this experiment?

4.The researchers working on happyase discovered that the compound STRESS is a potent inhibitor of happyase. A kinetics study of happyase in the presence of         1 nM STRESS, gives the following data:

[SAD] (uM)                V₀ (uMs^-1)      

20                    1.7 x 10³

40                   2.7 x 10³

60                    3.3 x 10³               

80                    3.7 x 10³

Which class of inhibitors does STRESS belong to?

This is the substrate concentration and the kinetic slopes from lab experiment. I'm suppose to construct a graph to estimate Km and Vmax (that's where I need help. How do I do that? Also, I'm suppose to construct another graph of "linear transfored" plot (lineweaver-burk or Eadie-hofstee plots) to determine Km and Vmax. HELP!

use the following information to determine if the intermolecular forces of isopropyl alcohol are greater or weaker than the intermolecular forces of water? the melting point of iso alcohol is about -90c and the boiling point is about 82c.

B. What happens to heat energy when it is not increasing the temperature of the substance in the beaker?

C.What occurs to the particles of a substance when the substance melts. why does this occur?

Use the data found in the appendix to determine the values for the O-Cl and the O-F bond energies. (heats of formation of OF₂ and OCl₂ are 24.7 and 80.37 kJ/mol, respectively)

solution, mL dissolved rock
1.              10
2.               10
3.              10
4.              10
5.               10
                 mL 0.100M sodium metasilicate added
0
1.00
2.00
4.00
20.00
total volume
50
50
50
50
50
Si Signal (mA)
0.235
0.500
0.768
1.250
5.202
A 10.000g sample of rock containing quartz as the sole source of silicon is dissolved and diluted to 1.000 +/- 0.010 liters and analyzed by the method of standard additions. What is the wt% of quartz in the rock?

Determine the pH of a solution that is 0.025 M C2H8N2 where Kb(C2H8N2) = 8.3 * 10−5?

The reaction F + G = H is at equilibrium, under standard conditions.

(a) Calculate ∆Go’ if [F] = 2mM, [G] = 3 mM, and [H] = 9 mM

(b) What happens to ∆Go’ if the solution is at equilibrium at twice this concentration of F?

(c) What happens to ∆Go’ if the solution is at equilibrium at twice this concentration of H?

(d) Does this violate LeChatelier’s Principle?

Consider the following chemical reaction: 2 CH4(g) 3 O2(g) 2 CO(g) 4 H2O(l) Select all the statements that are correct:

The reaction should become more product favored at high temperatures

The reaction should become more product favored at low temperatures

The reactants can adopt a larger number of configurations than the products

The potential energy of the reactants is higher than the potential energy of the products

The potential energy of the reactants is lower than the potential energy of the products

The reactants can adopt a fewer number of configurations than the products

Please show work.

A. For single reactant: observe the change in rate for a change in initial reactantconcentration.

The table above shows three independent reactions (not related to each other). What are the rate laws for each of thesereactions?

B. For multiple reactants: Find the reaction order for each reactant separately.

For the table above, find the rate law and the rate constant. What is the overall order of the reaction?

ErmTR is an enzyme found in S. pyogenes strains that are resistant to erythromycin. While working in the lab one afternoon you discover a small molecule (C23) that inhibits the activity of ermTR. To assess the type of inhibitor you discovered, you set up reactions with 6 different concentrations of erythromycin and varying amounts of the C23 small molecule you discovered. To each reaction you add 2 x 10-12 mol of ermTR (total reaction volume: 15 mL) and measure the initial velocities, Vo, by spectrophotometry. You obtained the following data:

Initial Velocity Vo (micromoles/min)

Display the data on a Lineweaver-Burk plot in order to extract the Km and Vmax values. You can
generate the plot by hand on the graph paper on the last page or use a program, such as Excel, to plot the data. Submit the plot you generate with your test answers.

b) What are the KM and Vmax values for each of the samples? Show your calculations and explain how you obtain the answer for the “no inhibitor” sample.

c) What type of inhibitor is C23? Explain your answer.

d) Calculate the turnover number and the specificity constant for the enzyme sample that lacks the inhibitor C23.

11) In the reaction of nitrogen gas with hydrogen gas to form ammonia gas, NH3, bow many grams of hydrogen are needed to react with two moles of nitrogen?

The boiling point of pure ethanol at 760. torr is 82.0 degrees celsius. What would bet the vapor pressure of ethanol over a solution containing 2.13 mole of nonvolatile hydrocarbon and 15.30 moles of ethanol at 85.9 degrees celsius?

Explain causes leading to the plastic straps on a lawn chair seat, break after it has been left in sunlight for a long period of time. What types of additive can be used to prolong the life of the straps?

I am designing a mass spectrometer and the last task asks to figure out a certain compound based on the ions the spectrometer has found. It gives an ion ratio of 108:218:432:540 for m/z ratio of 16:14:12:1. I know the compound is supposed to be caffiene which has a formula of c8h10n4o2. Dividing the number of ions by 54 gives me the appropriate chemical formula, but I do not know the reasoning behind why I would divide by 54. I was wondering if someone could explain to me, based on the number of ions detected, how I would arrive to the chemical formula for caffeine?