Describe the Applications, Reagents, and Procedure of the Southern blot technique. Add bibliography.

Describe the Applications, Reagents, and Procedure of the Western blot technique. Add bibliography.

23. KCN is insoluble in benzene but in the presence of 18-crown-6 the nucleophilic substitution reactions take place very fast using CNas nucleophile- Justify with mechanism.

24. Arylamines and heterocyclic aromatic amines are considerably less basic than alkylamines — Justify.

25. p-Anisidine is more basic than aniline — Justify with appropriate drawings.

calculate the ph of .010 M sodium acetate

What will the molarity be of the resulting solutions made by mixing the following? Assume that volumes are additive.

a)116 mL of 3.6 M H₃PO₄ with 776 mL of H₂O

(b) 219 mL of 0.39 M Na₂SO₄ with 737 mL of H₂O

(c) 75 mL of 0.50 M HNO₃ with 75 mL of 1.5 M HNO₃

A) A solution of Ba(OH)₂ has a molarity of 0.00617 mol/L. Calculate the final pH of the solution.

B) Consider the reaction: CaBr₂(s) → Ca(s) + Br₂(l)

Which of the following statements is correct? Select all that apply.

Bromide Ion is reducing agent

NOT a spontaneous reaction

Ca is oxidized

Br is Oxidized

Br is Reduced

Ca is Reduced

Calcium Ion is the Reducing Agent

IS a spontaneous reaction

Br ion is the oxidizing agent.

Ca ion is the oxidizing agent.

C) What would cause the equilibrium to shift left CH3COOH(aq) + H2O(l) ⇌ CH3COO–(aq) + H3O+(aq).

Add HCL

pH increasing

Add acetic acid

Acetate Ion addition

D) 3 A₂ (g) + 2 Z₃ (g) ↔ 3 Z₂A₂ (g)
The equilibrium concentrations are: A₂ = 0.061 mol/L ; Z₃ = 0.769 mol/L ; Z₂A₂ = 0.245 mol/L.
Calculate the value of the equilibrium constant at 345^oC.

E)

Which of the following equations represents H₂BO₃⁻ acid

Sketch a general Lineweaver-Burke plot that illustrates the kinetic behavior of a non-allosteric enzyme. Identify on your plot the features that allow one to determine Vmax and KM for the enzyme.

How would one use a Lineweaver-Burke plat of enzyme kinetic data to identify an inhibitor's mode of action?

For the following systems at equilibrium A:2NOCl(g)⇌2NO(g)+Cl2(g) B: H2(g)+I2(g)⇌2HI(g) classify these changes by their effect.

Drag the appropriate items to their respective bins.

Bins: leftward shift, no shift, rightward shift

Items: system A Decrease container size, system A Increase container size, system b Increase container size, system B Decrease container size

Is more than one shape possible for the caproic acid molecule? If you built a model of stearic acid by extending the carbon chain of caproic acid to18 carbons, would it have more, less, or the same number of possible shapes as the number of shapes for caproic acid? Can you write a general rule that relates the length of a fatty acid chain (carbon skeleton, backbone) to the number of shapes possible?

Unrelated but... Do the open chain and closed ring forms of glucose have the same molecular weight?

You need to prepare an acetate buffer of pH 5.55 from a 0.654 M acetic acid solution and a 2.92 M KOH solution. If you have 530 mL of the acetic acid solution, how many milliliters of the KOH solution do you need to add to make a buffer of pH 5.55 ? The p K a of acetic acid is 4.76.

Constant-volume calorimeters are sometimes calibrated by running a combustion reaction of known ΔE and measuring the change in temperature. For example, the combustion energy of glucose is 15.57 kJ/g. When a 2.000 g sample of glucose burns in a constant volume calorimeter, the calorimeter temperature increases from 21.45 to 23.34°C. Find the total heat capacity of the calorimeter (in kJ/K).

Consider a crystallization of sulfanilamide in which 10ml of hot 95% ethyl alcohol is added to 0.10g of impure sulfanilamide. After the solid has dissolved, the solution is cooled to room temperature and then placed in an ice water bath. No crystals form, even after scratching with a glass rod. explain why this crystallization failed. What would you have to do at this point to make the crystallization work??

explain how PLA is biodegradable?

Draw several ammonia molecules in Lewis structure, then add in some hydrogen bonds where appropriate; label the atoms that are involved in the hydrogen bonds as partially positive or negative.