You now plan to amplify a 770 bp region of this gene,
highlighted in grey below, which includes the full coding sequence
plus some flanking regions. Design a primer set (forward &
reverse primers of 20 nucleotide each) that will allow you to
amplify only the sequence highlighted in grey in your PCR reaction.
(2)
AGCCAACTCAAGGATTTAACAGCGACAACAACGCATTACACCTTTTCTGGCAGGACGGAGATAAAGTCTT
ACCGCTTGAGCGCAAAGAGCTCCTTGGCCAATTATTACTCGACGAGATCGTGACCCGTTATGATGAAAAA
AATCGACGTTAAGATTCTGGACCCGCGCGTTGGGAAGGAATTTCCGCTCCCGACTTATGCCACCTCTGGC
TCTGCCGGACTTGACCTGCGTGCCTGTCTCAACGACGCCGTAGAACTGGCTCCGGGTGACACTACGCTGG
TTCCGACCGGGCTGGCGATTCATATTGCCGATCCTTCACTGGCGGCAATGATGCTGCCGCGCTCCGGATT
GGGACATAAGCACGGTATCGTGCTTGGTAACCTGGTAGGATTGATCGATTCTGACTATCAGGGCCAGTTG
ATGATTTCCGTGTGGAACCGTGGTCAGGACAGCTTCACCATTCAACCTGGCGAACGCATCGCCCAGATGA
TTTTTGTTCCGGTAGTACAGGCTGAATTTAATCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGG
CGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACGTCATAACATAGCCGCAAACAT
TTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGCA
GAAAAACAAACTGCGAAAAGGAACCGTCGCGAGGAAATACTTCAGTCTCTGGCGCTGATGCTGGAATCCA
GCGATGGAAGCCAACGTATCACGACGGCAAAACTGGCCGCCTCTGTCGGCGTTTCCGAAGCGGCACTGTA
TCGCCACTTCCCCAGTAAGACCCGCATGTTCGATAGCCTGATTGAGTTTATCGAAGATAGCCTGATTACT
(note: primers should not extend beyond the grey region)
Fp:
Rp:
NB:...